작성일 : 12-05-06
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Atto-conjugated 2ndary antibodies
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글쓴이 :
운영자
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| Atto-conjugated Secondary Antibodies New generation antibodies: Superior photo-stability & strong fluorescence!
매우 안정한 dyes에 conjugate한 항체로, 매우 높은 affinity를 갖고 있습니다. 이미 독일과 유럽의 연구자들 사이에 이미 정평이 있으면서도 가격은 상대적으로 저렴합니다.
The quality of secondary antibodies is essential in producing superior results in immunofluorescence.
Hypermol offers secondary antibodies conjugated to the novel, high quality Atto dyes for detecting primary
antibodies.
Atto-labeled antibodies belong to the new generation of fluorescent antibodies characterized by their
exceptional fluorescence intensity and superior photostability. For this reason they are suited for all kinds of
immunofluorescence microscopy and mostly also for high resolution techniques.
Compared to established fluorescent antibodies, Atto antibodies show a remarkably prolonged fluorescence,
as a result of the outstanding features of the dyes, as demonstrated by two examples:
1. Atto 647N fluoresces in aqueous solution twice as strong as Cy5TM.
2. under identical conditions Atto 647N lasts up to 100 times longer than dyes like Cy5TMand Alexa 647TM.
Atto-conjugated IgGs from Hypermol are affinity purified and quality-control tested by UV-VIS spectroscopy
and immunocytochemistry. They come as convenient kits with a well characterized, epitope mapped actin
antibody for reference, double staining or ligand analysis, with a detailed description in the product
documentation of each kit.
Anti-Mouse IgG Atto Highly specific secondary antibodies - strong & long lasting fluorescence!
| | Anti-Mouse IgG Atto390 (H+L), Antibody produced in goat
Description | Product name | | Goat anti-mouse IgG Atto390 | | Target species | | Mouse | | Description | | Atto-labeled secondary antibodies belong to the new generation of fluorescent
antibodies characterized by their exceptional fluorescence intensity and superior
photostability. Goat anti-mouse Atto390 is specifically suited for high resolution
microscopy, due to the high photostability and strong fluorescence quantum yield.
For convenient reference, Fluorescent Antibody Kits contain gratis a primary epitope
mapped actin antibody of high performance also on western blots. | | Specifications | | Goat anti-mouse IgG Atto390 (H+L), Immunofluorescence: 1:500-1:1500,
Degree of labeling (DOL) : 2-9, Unconjugated dye ≤5% of total fluorescence.
For further information please see DataSheet. | | Optical properties | | λex 390nm, λem 479nm |
Properties | Form | | Liquid, ready-to-use | | Concentration | | 2mg/ml | | Content | | 1mg (2mg/ml) goat-anti mouse IgG (H+L) Atto390*
50µg (1mg/ml) monoclonal anti-actin*
Product documentation
Certificate of Analysis
| | Buffer | | *in 50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, 10 mM sodium
azide, pH 7.4. | | Clonality | | Polyclonal | | Isotype | | IgG | | Purification notes | | Column affinity purified | | Storage condition | | Store as glycerol stock at -20°C. Avoid repeated freeze / thaw cycles. | | Shipping condition | | Shipped as liquid on ice within EU. Upon delivery prepare aliquots and store at -20°C.
To preserve the high quality, the product is shipped lyophilized for shipping times
longer than 2 days, including a glycerol buffer for convenient reconstitution. |
| Anti-Mouse IgG Atto488 (H+L), Antibody produced in goat
Description | Product name | | Goat anti-mouse IgG Atto488 | | Target species | | Mouse | | Description | | Atto-labeled secondary antibodies belong to the new generation of fluorescent
antibodies characterized by their exceptional fluorescence intensity and superior
photostability. Goat anti-mouse Atto390 is specifically suited for high resolution
microscopy, due to the high photostability and strong fluorescence quantum yield.
For convenient reference, Fluorescent Antibody Kits contain gratis a primary epitope
mapped actin antibody of high performance also on western blots. | | Specifications | | Goat anti-mouse IgG Atto488 (H+L), Immunofluorescence: 1:500-1:1500,
Degree of labeling (DOL) : 2-9, Unconjugated dye ≤5% of total fluorescence.
For further information please see DataSheet. | | Optical properties | | λex 488nm, λem 523nm |
Properties | Form | | Liquid, ready-to-use | | Concentration | | 2mg/ml | | Content | | 1mg (2mg/ml) goat-anti mouse IgG (H+L) Atto488*
50µg (1mg/ml) monoclonal anti-actin*
Product documentation
Certificate of Analysis | | Buffer | | *in 50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, 10 mM sodium azide, pH 7.4. | | Clonality | | Polyclonal | | Isotype | | IgG | | Purification notes | | Column affinity purified | | Storage condition | | Store as glycerol stock at -20°C. Avoid repeated freeze / thaw cycles. | | Shipping condition | | Shipped as liquid on ice within EU. Upon delivery prepare aliquots and store at -20°C.
To preserve the high quality, the product is shipped lyophilized for shipping times
longer than 2 days, including a glycerol buffer for convenient reconstitution. |
| Anti-Mouse IgG Atto514 (H+L), Antibody produced in goat
Description | Product name | | Goat anti-mouse IgG Atto514 | | Target species | | Mouse | | Description | | Atto-labeled secondary antibodies belong to the new generation of fluorescent
antibodies characterized by their exceptional fluorescence intensity and superior
photostability. Goat anti-mouse Atto514 is specifically suited for high resolution
microscopy, due to the high photostability and strong fluorescence quantum yield.
For convenient reference, Fluorescent Antibody Kits contain gratis a primary epitope
mapped actin antibody of high performance also on western blots. | | Specifications | | Goat anti-mouse IgG Atto514 (H+L), Immunofluorescence: 1:500-1:1500,
Degree of labeling (DOL) : 2-9, Unconjugated dye ≤5% of total fluorescence.
For further information please see DataSheet. | | Optical properties | | λex 514nm, λem 533nm |
Properties | Form | | Liquid, ready-to-use | | Concentration | | 2mg/ml | | Content | | img (2mg/ml) goat-anti mouse IgG (H+L) Atto514*
50µg (1mg/ml) monoclonal anti-actin*
Product documentation
Certificate of Analysis | | Buffer | | *in 50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, 10 mM sodium
azide, pH 7.4. | | Clonality | | Polyclonal | | Isotype | | IgG | | Purification notes | | Column affinity purified | | Storage condition | | Store as glycerol stock at -20°C. Avoid repeated freeze / thaw cycles. | | Shipping condition | | Shipped as liquid on ice within EU. Upon delivery prepare aliquots and store at -20°C.
To preserve the high quality, the product is shipped lyophilized for shipping times
longer than 2 days, including a glycerol buffer for convenient reconstitution. |
Table of dyes and light sources | Secondary Antibodies series Anti-Mouse IgG Atto (본 화면에서 소개 중 입니다)
Anti-Mouse IgG F(ab) Atto
Anti-Mouse IgG F(ab')2 Atto
Anti-Mouse IgM Atto
Anti-Rabbit IgG Atto
Anti-Rabbit IgG F(ab) Atto
Ordering informations
| Catalog No. | Product Name | Size | | 2101-1MG (PDF) | Anti-Mouse IgG Atto390 (H+L) | kit | | 2102-1MG (PDF) | Anti-Mouse IgG Atto488 (H+L) | kit | | 2103-1MG (PDF) | Anti-Mouse IgG Atto514 (H+L) | kit | | 2104-1MG (PDF) | Anti-Mouse IgG Atto532 (H+L) | kit | | 2105-1MG (PDF) | Anti-Mouse IgG Atto550 (H+L) | kit | | 2107-1MG (PDF) | Anti-Mouse IgG Atto565 (H+L) | kit | | 2106-1MG (PDF) | Anti-Mouse IgG Atto594 (H+L) | kit | | 2108-1MG (PDF) | Anti-Mouse IgG Atto647N (H+L) | kit | | 2110-1MG (PDF) | Anti-Mouse IgG Atto700 (H+L) | kit | | 2111-1MG (PDF) | Anti-Mouse IgG Atto740 (H+L) | kit |
Catalog of Atto secondary antibodies (PDF)
▣ 관련 페이지 ; HYPERMOL EK
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