작성일 : 18-06-25
Cites AlbuSorb™ for Serum Exosome Proteomic Analysis
 글쓴이 : 운영자
 

 

클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

 

 

News Release

 

Journal Article Cites AlbuSorb™ for Serum Exosome Proteomic Analysis

MONMOUTH JUNCTION, NJ, May 14, 2018 -- Biotech Support Group reports on a recent journal article using

AlbuSorb™ to improve serum exosome purification for subsequent proteomic analysis.

     

The citation is:

Chettimada, Sukrutha, et al. "Exosome markers associated with immune activation and oxidative stress in HIV patients on antiretroviral therapy." Scientific Reports 8.1 (2018): 7227.

 

The authors investigated the role that exosomes play in HIV pathogenesis, as little is known about exosome

cargo in relation to immune responses and oxidative stress. For this, plasma exosomes in HIV patients and

their relationship to immunological and oxidative stress markers were characterized by electron microscopy,

nanoparticle tracking, immunoblotting, and LC-MS/MS proteomics. For LC-MS/MS proteomics, the article

states “To obtain purified exosome fractions for proteomic analysis,… albuminwas depleted with 2 rounds of

albumin depletion using AlbuSorb™ - Albumin Depletion Kit (Biotech Support Group)”. Using untargeted

proteomic analysis, Exosomal Notch4 was increased in HIV-positive subjects versus controls and correlated

with immune activation markers. The results from this study suggest that exosomes in antiretroviral therapy-

treated HIV patients carry proteins related to immune activation and oxidative stress, have immunomodulatory

effects on myeloid cells, and may have pro-inflammatory andredox effects during pathogenesis.

“This reference shows that while exosomes are large hydrophobic vesicles, separating them from

surrounding protein content, can be difficult. Nevertheless, AlbuSorb™ is demonstrated here to be useful in

depleting albumin and enriching exosomes. Thus LC-MS/MS proteomic analysis of the exosomes is not

compromised by interference from serum albumin.” states Swapan Roy, Ph.D., President and Founder of

Biotech Support Group.

 

For more information visit: AlbuSorb at

https://www.biotechsupportgroup.com/AlbuSorb-Albumin-Depletion-Kit-p/a185.htm

  

 

▣ 관련 페이지 ; Biotech Support Group