Journal Article Cites Cleanascite™ To Deplete Extracellular Vesicles
MONMOUTH JUNCTION, NJ, June 14, 2018 -- Biotech Support Group reports on a recent journal article
describing the simplicity and efficiency of their lipid binding sample preparation technology for depleting the
influence of extracellular vesicles – also known as EVs or exosomes, on cell-cell communication.
The citation is:
Nguyen, Doan C., et al. "Extracellular vesicles from bone marrow-derived
mesenchymal stromal cells support ex vivo survival of human antibody secreting
cells." Journal of extracellular vesicles 7.1 (2018): 1463778.
Bone marrow-derived mesenchymal stromal cells (MSC) have been shown to support human antibody
secreting cells (ASC) survival ex vivo. Extracellular vesicles from bone marrow-derived mesenchymal
stromal have novel mechanisms of cell-cell communication over short and long distances, but whether the
crosstalk between these cell interactions can occur via extracellular vesicles is not known.
Thus, the study investigated the role of extracellular vesicles (EVs) in antibody secreting cell survival and
IgG secretion. The article states “To understand whether disrupting the lipid plasma membrane that upsets
the integrity of the EVs would compromise the survival activity of either the non-irradiated or irradiated
secretomes, …We then cultured ASC with conventional media (vehicle), secretome from irradiatedMSC, or
secretome from irradiated MSC that had been pretreated with the lipid-disrupting agent Cleanascite ,
which is known not to alter protein functionality . Cleanascite-treatment of the secretome dramatically
reduced ASC functional survival, … Similar reductions were also noted with the secretome of non-irradiated
MSC when treated with Cleanascite … These results demonstrate that lipid-membrane bodies, such as EVs,
could mediate important ASC survival factors within the MSC secretome.”
“This reference shows that Cleanascite™ can simply and efficiently bind the lipid membrane complex
necessary for studying extracellular vesicle communication between cells. This demonstrates the advantage
of using an aqueous suspension product with no solvents, to deplete lipid associated factors that may
influence cell response investigations. The exosome depletion is quite impressive” states Swapan
Roy,Ph.D., President and Founder of Biotech Support Group.
For more information visit: Cleanascite™ Lipid Removal Reagent and Clarification,