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[Emfret Analytics] Antibodies for In Vivo Mouse Platelet Labeling
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Antibodies for In Vivo Mouse Platelet Labeling

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This antibody preparation contains a rat IgG derivative against the GPIbb subunit of the murine

platelet/megakaryocyte-specific GPIb-V-IX complex. The modified antibody has been optimized for the easy

and stable in vivo labelling of circulating platelets in mice. At the recommended concentration (0.1 ¥ìg/g body

weight), X-488 / X-649 is non-cytotoxic and does not interfere with platelet adhesion and aggregationin vivo.

Also, X-488 (X-649 at the recommended concentration) does not alter platelet adhesion on collagen/von

Willebrand factor in vitro. In vivo platelet labeling has been used for intravital microscopical analysis of

platelet involvement in pathological processes, such as thrombosis.1,2

 

 

In vivo fluorescence microscopy of arterial thrombus formation using X488

 

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An anesthetized mouse (15 g) received 1.5 ¥ìg X488 in sterile PBS intravenously and the mesenteric artery

was gently exteriorized throug a midline abdominal incision. Arterioles (35-60 ¥ìm diameter) were visualized

with a Zeiss Axiovert 200 inverted microscope (x10) equipped with a 100 W HBO fluorescent lamp source and

a CCD camera (CV-M300). Injury was induced by topical application of a 3 mm2 filterpaper saturated with

FeCl3for 10 s. Adhesion and aggregation of fluoresently labeled platelets in arterioles were monitored until

complete occlusion occurred (blood flow stopped for > 1 min). Note that adhesion of single platelets can be

detected at t=5 min.

 

 

References

1. Falati et al. (2002) Real-time in vivo imaging of platelets, tissue factor and fibrin during arterial thrombus

   formation in the mouse. Nature Medicine 8, 1175-1181

2. Grosse et al., (2007): An EF hand mutation in Stim1 causes premature platelet activation and bleeding in

   mice. J Clin Invest. 117, 3540-3550

 

 

 

Ordering informations

    

Catalog No.

Product Name

Size

X488 (PDF)

Antibodies for In Vivo Mouse Platelet Labeling, Anti-GPIb, Rat IgG, DyLight 488

100 µg

X649 (PDF)

Antibodies for In Vivo Mouse Platelet Labeling, Anti-GPIb, Rat IgG, DyLight 649

100 µg

 

 

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