Antibodies for In Vivo Mouse Platelet Labeling

마우스 혈소판 연구용 항체로 정맥 내 투여 후 혈소판이 표지되며, 세포 독성이 없고 혈소판 접착 및 응집에

영향이 없습니다.

This antibody preparation contains a rat IgG derivative against the GPIbb subunit of the murine

platelet/megakaryocyte-specific GPIb-V-IX complex. The modified antibody has been optimized for the easy

and stable in vivo labelling of circulating platelets in mice. At the recommended concentration (0.1 μg/g body

weight), X-488 / X-649 is non-cytotoxic and does not interfere with platelet adhesion and aggregationin vivo.

Also, X-488 (X-649 at the recommended concentration) does not alter platelet adhesion on collagen/von

Willebrand factor in vitro. In vivo platelet labeling has been used for intravital microscopical analysis of

platelet involvement in pathological processes, such as thrombosis.^1,2

In vivo fluorescence microscopy of arterial thrombus formation using X488

An anesthetized mouse (15 g) received 1.5 μg X488 in sterile PBS intravenously and the mesenteric artery

was gently exteriorized throug a midline abdominal incision. Arterioles (35-60 μm diameter) were visualized

with a Zeiss Axiovert 200 inverted microscope (x10) equipped with a 100 W HBO fluorescent lamp source and

a CCD camera (CV-M300). Injury was induced by topical application of a 3 mm² filterpaper saturated with

FeCl₃for 10 s. Adhesion and aggregation of fluoresently labeled platelets in arterioles were monitored until

complete occlusion occurred (blood flow stopped for > 1 min). Note that adhesion of single platelets can be

detected at t=5 min.

References

1. Falati et al. (2002) Real-time in vivo imaging of platelets, tissue factor and fibrin during arterial thrombus

   formation in the mouse. Nature Medicine 8, 1175-1181

2. Grosse et al., (2007): An EF hand mutation in Stim1 causes premature platelet activation and bleeding in

   mice. J Clin Invest. 117, 3540-3550