Antibodies for In Vivo Mouse Platelet Labeling
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This antibody preparation contains a rat IgG derivative against the GPIbb subunit of the murine
platelet/megakaryocyte-specific GPIb-V-IX complex. The modified antibody has been optimized for the easy
and stable in vivo labelling of circulating platelets in mice. At the recommended concentration (0.1 ¥ìg/g body
weight), X-488 / X-649 is non-cytotoxic and does not interfere with platelet adhesion and aggregationin vivo.
Also, X-488 (X-649 at the recommended concentration) does not alter platelet adhesion on collagen/von
Willebrand factor in vitro. In vivo platelet labeling has been used for intravital microscopical analysis of
platelet involvement in pathological processes, such as thrombosis.1,2
In vivo fluorescence microscopy of arterial thrombus formation using X488

An anesthetized mouse (15 g) received 1.5 ¥ìg X488 in sterile PBS intravenously and the mesenteric artery
was gently exteriorized throug a midline abdominal incision. Arterioles (35-60 ¥ìm diameter) were visualized
with a Zeiss Axiovert 200 inverted microscope (x10) equipped with a 100 W HBO fluorescent lamp source and
a CCD camera (CV-M300). Injury was induced by topical application of a 3 mm2 filterpaper saturated with
FeCl3for 10 s. Adhesion and aggregation of fluoresently labeled platelets in arterioles were monitored until
complete occlusion occurred (blood flow stopped for > 1 min). Note that adhesion of single platelets can be
detected at t=5 min.
References
1. Falati et al. (2002) Real-time in vivo imaging of platelets, tissue factor and fibrin during arterial thrombus
formation in the mouse. Nature Medicine 8, 1175-1181
2. Grosse et al., (2007): An EF hand mutation in Stim1 causes premature platelet activation and bleeding in
mice. J Clin Invest. 117, 3540-3550
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