The multipurpose antibody for analysis of GPVI
The monoclonal antibody JAQ1 reacts with the platelet collagen receptor GPVI. On the one hand, it inhibits
collagen-induced aggregation of mouse platelets, whereas cross-linking of JAQ1 by a secondary antibody
induces platelet activation and aggregation.
JAQ1 can be used for immunoprecipitation, immunohistochemical analysis of acetone-fixed frozen sections,
immunofluorescent staining, and Western blot analysis under non-reducing condtions.
JAQ1 recognizes GPVI in Western blot analysis
After separation of mouse platelet lysate by SDSPAGE (non-reducing conditions) and transfer to PVDF
membrane, the membrane was incubated with JAQ1 (1-3 μg/ml) and bound antibody was visualized with
HRP-conjugated anti-rat IgG and ECL.
Detection of GPVI on mouse platelets
Mouse blood was diluted 1:20 and 25 μl of this dilution were stained with 5 μl control IgG-FITC (emfret
Analytics, P190-1, black line) or JAQ1-FITC (shaded area) for 15 min at RT and analyzed directly.
Platelets were gated by FSC/SSC characteristics.
1. Clemetson JM, Polgar J, Magnenat E, et al. (1999) The platelet collagen receptor
glycoprotein VI is a member of the immunoglobulin superfamily closely related to FcalphaR
and the natural killer receptors. J Biol Chem. 274(41):29019-24
2. Nieswandt B, Watson SP. (2003) Platelet-collagen interaction: is GPVI the central
receptor? Blood. 102(2):449-61.
3. Nieswandt B, et al. (2000): Expression and function of the mouse collagen receptor
glycoprotein VI is strictly dependent on its association with the FcRgamma chain. J Biol