The multipurpose antibody for analysis of GPVI

The monoclonal antibody JAQ1 reacts with the platelet collagen receptor GPVI. On the one hand, it inhibits

collagen-induced aggregation of mouse platelets, whereas cross-linking of JAQ1 by a secondary antibody

induces platelet activation and aggregation.

JAQ1 can be used for immunoprecipitation, immunohistochemical analysis of acetone-fixed frozen sections,

immunofluorescent staining, and Western blot analysis under non-reducing condtions.

JAQ1 recognizes GPVI in Western blot analysis

After separation of mouse platelet lysate by SDSPAGE (non-reducing conditions) and transfer to PVDF

membrane, the membrane was incubated with JAQ1 (1-3 μg/ml) and bound antibody was visualized with

HRP-conjugated anti-rat IgG and ECL.

Detection of GPVI on mouse platelets

Mouse blood was diluted 1:20 and 25 μl of this dilution were stained with 5 μl control IgG-FITC (emfret

Analytics, P190-1, black line) or JAQ1-FITC (shaded area) for 15 min at RT and analyzed directly.

Platelets were gated by FSC/SSC characteristics.

References

1. Clemetson JM, Polgar J, Magnenat E, et al. (1999) The platelet collagen receptor

   glycoprotein VI is a member of the immunoglobulin superfamily closely related to FcalphaR

   and the natural killer receptors. J Biol Chem. 274(41):29019-24

2. Nieswandt B, Watson SP. (2003) Platelet-collagen interaction: is GPVI the central

   receptor? Blood. 102(2):449-61.

3. Nieswandt B, et al. (2000): Expression and function of the mouse collagen receptor

   glycoprotein VI is strictly dependent on its association with the FcRgamma chain. J Biol

   Chem. 275(31):23998-4002