Minute¢â Native Protein Precipitation Kit from Saliva
Human saliva is a readily available source for identifying biomarkers for diagnosis of local and systemic
diseases. Proteins in saliva contain biomarkers that can be identified by proteomics for the diagnosis of
many diseases such as Sjögren¡¯s syndrome, squamous cell carcinoma, diabetes, breast cancer, dental
caries, periodontitis, systemic sclerosis and bleeding oral cavity etc. Historically, the most common method
for extracting proteins from saliva is trichloroacetic acid (TCA) precipitation.Although relatively simple, rapid
and effective, TCA precipitation suffers a major drawback e.g. the precipitated proteins are denatured and
insoluble in detergent-free aqueous solution. That limits the downstream application of extracted proteins.
By contrast, the native protein extraction kit is designed to overcome the disadvantage of TCA method using
a propitiatory protein precipitation reagent that extracts native proteins from saliva. The method is also very
simple and rapid with high yield. The precipitatedproteins can be readily dissolved in detergent-free aqueous
solutions.


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