The detergent-free nuclei isolation kit is designed to rapidly isolate intact nuclei from animal cultured cells or

tissues (fresh or frozen). Intact nuclei can be isolated from the samples using proprietary spin-column-

based technologies in less than 20 min without using tissue homogenizer and any detergents. Traditional

method for nuclei isolation involves the use of non-ionic detergent, which has tendency to cause unwanted

nuclear aggregation. It is unclear why some nuclei are aggregatedwhile others are not. It is also unknown

whether non-aggregated nuclei are unbiased representation of the whole nuclei population. Detergents also

have the potential to damage the nuclear envelope resulting in leakage of nuclear matrix materials. For some

cell/tissue types, nuclear envelop proteins could also be stripped off by the detergents. MinuteTM Detergent-

Free Nuclei Isolation Kit provides a whole new way for nuclei isolation in contrast to traditional methods.

How it works: cells/tissues are first sensitized by buffer A before passing through the proprietary filter in a

zigzag manner when high-speed centrifugal force is applied. The cells are ruptured when passing through

the filter leaving intact native nuclei in the flow through. The nuclei are separated from other small cell debris

by low speed centrifugation using the proprietary buffer B. The native and intact nuclei isolated can be used

for a variety of downstream applications that includebut not limited to: FACS analysis, single nucleus

analysis (such as RNA-seq and ATAC-seq), immunofluorescence staining, cell cycle analysis and/or

apoptosis research.

NOTE: We also offer the following single nucleus isolation kits with much cleaner background if the presence

of detergent is not a concern:

SN-047 Minute™ Single Nucleus Isolation Kit for Tissues/Cells

BN-020 Minute™ Single Nucleus Isolation Kit for Neuronal Tissues/Cells

AN-029 Minute™ Nuclei and Cytosol Isolation Kit for Adipose Tissues/Cultured Adipocytes