SeleCTEV™ DNA Enrichment Kit

Puri cation of circulating free DNA (cfDNA) & tumor-derived EVs DNA

SeleCTEV™ DNA Enrichment Kit allows the selective purification of circulating free DNA (cfDNA) and tumor-

derived extracellular vesicles (EVs) DNA from plasma. The isolation is based on Exosomics’proprietary

peptide affinity method. Enables better downstream analytical performance.

Applications

• Purifies and concentrates DNA from tumor-derived EVs and cfDNA from biofluids that contain low

  abundance fragmented DNA

• For any liquid biopsy sample that requires analysis of DNA at low amount allelic frequency (AF)

Advantages

• Highly efficient pre-analytical method to harvest tumor DNA from biofluids

• Spin column technology without use of organic solvents

• Flexible sample input

Mutation Recovery & Enrichment

Mutation-bearing EVs and cfDNA were spiked alone and together into healthy donor plasma and processed

with SeleCTEV™ and Competitor Q to obtain DNA. SeleCTEV™ isolated more mutation thank Competitor Q

from both biological sources, suggesting that SeleCTEV™ is a more efficient way to isolate EVs and cfDNA

than Competitor Q.

Case Study: Metastatic Melanoma Patients

SeleCTEV™ and Competitor Q were used for monitoring BRAFV600E levels in the plasma of BRAF inhibitor-

treated MM patients. In patient #1 disease progression (DP) occurred within 3 months and was associated to

rebounding levels of circulating BRAFV600E and unfavorable prognosis. In patient #2 no clinical evidence of

disease progression was observed at later time points, and mutant gene copies remained low or

undetectable in plasma.

 Data Sheet (PDF)