HOOK™ GST Protein Purification (Bacteria)

HOOK™ GST Protein Purification kit allows for the purification of soluble, GST tagged protein from bacterial

cultures.  The bacteria are first lysed with Bacterial PE LB™ and PE LB™-Lysozymeto release total soluble

protein,whilst maintaining the structure and activity of the protein.The GST tagged protein is purified by

passing clarified lysate through prepacked columns.

Bacterial- PELB™ kit has been developed for the extraction of soluble proteins from bacterial cells. It is a

proprietary improvement on the lysozyme based lysis, which allows extraction of soluble proteins and

concurrent removal of nucleic acids (DNA & RNA) released during cell lysis. The Bacterial-PE LB™ lysis

eliminates viscosity build-up, allowing effective clarification with lower centrifugal force.

HOOK™ GST Protein Purification kit is optimized to yield 10mg/250ml culture of soluble GST tagged protein,

with a purity of 80-90%, dependent on expression levels, resin type, conformation and solubility

characteristics of the protein.

For smaller scale, rapid GST protein isolation from bacteria we recommend our spin format kits.

Features;

• Isolate >10mg GST tagged proteins from ~250ml bacteria

• No sonication required, supplied with lysis buffer and enzyme

Application;

• For the isolation of GST tagged recombinant proteins from bacteria

 Instruction sheet (PDF)