BroadPharm
 
작성일 : 22-03-28
[BroadPharm] MagicLink™ Alkaline Phosphatase Antibody Labeling Kit

 

MagicLink™ Alkaline Phosphatase Antibody Labeling Kit

 

   클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

A typical flow chart of AP-antibody conjugation by MagicLink™ technology.

 

Alkaline phosphatase (AP) can be conjugated to antibody/protein by thiol-maleimide and hydrazone

coupling approaches. The thiol-maleimide approach is commonly performed with a bi- functional linker

(such as SMCC). However, SMCC-modified protein is extremely unstable and often self-reactive since it

often contains both amine and thiol groups that cause significant amounts of homo-crosslinking. The thiol-

maleimide linkage is exchangeable when other thiol groups are present. Inthe hydrazone coupling approach,

the conjugation reaction is based on a reaction between hydrazine and aldehyde. This method is low

yielding although a catalyst is used. The size of the molecule and inaccessibility of the functional group

contribute to low yield. In addition, linkage stability is a concern.

BroadPharm's MagicLink™ technology solves all the problems by combining PEG and proprietary

crosslinking chemistry. MagicLink™chemistry and kit do not need TCEP, DTT, or catalysts at all!

First, antibody reacts with MAGIC NHS ester to yield the MAGIC activated antibody which instantly reacts

with LINK preactivated AP to yield antibody-AP conjugate with a stable ring linkage.

 

Features;

• Novelty: 3rd generation protein crosslink technology

• Yield: conjugation efficiency around 95-100%

• Rate:instant reaction; the whole process less than 1 hour

• Linkage stability: most stable covalent bonded conjugates on the market

• Consistence: good reproducibility from batch to batch

• Flexible: use your own AP and antibody at your desired ratio

• Shelf life: long, stable, freeze dried ship at ambient temperature

• Antibody selection: any proteins/antibody, antibody fragment

• Post-conjugation clean-up step not necessary

• AP activity:  4200 U/mg, high specific activity grade

 

  클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

Alkaline phosphatase conjugated

antibody; reducing conditions. Lanes

M) protein marker, 1) alkaline

phosphosphatase (calf intestine), 2)

unconjugate antibody, 3) antibody

conjugated with alkaline phosphatase.

 

Application;

Alkaline Phosphatase Labeling Kit is used mainly for the preparation of alkaline phosphatase antibodies and

other proteins in immunological techniques such as ELISA, Western blotting, and immunohistochemistry.

 

클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

 

Consists of;

• MAGIC NHS (MW ~900)

• LINK activated AP

• Reaction buffer

• Protein concentrator

 

  Instruction sheet (PDF)

  

Ordering informations;

  

Catalog No.

Product Name

Size

BP-50090

MagicLink™ Alkaline Phosphatase Antibody Labeling Kit

1 x 100 ug

BP-50091

3 x 100 ug

BP-50092

1 x 1 mg

 

 

▣ 관련 페이지 ; Broadpharm