Li-Resin swells more homogeneously in solvents commonly used for solid phase synthesis and also
excellently in aqueous medium.
The swellability profile was determined using common solvents for solid phase synthesis. The results were
collected into one graph in comparison with the data of polystyrene (PS) resin and polyethylene glycol
(PEG) resin. Unlike the PS resin which is poorly solvated in acetonitrile, DMSO, methanol, trifluoroacetic
acid, and water, and unlike the PEG resin which is extremely solvated in trifluoroacetic acid, Li-Resin is
more homogeneous among the solvents tested. This is remarkably advantageous for solid phase synthesis
because the resin bed of Li-Resin may not expand and shrink dramatically due to the solvent change during
a synthesis cycle, providing a more stable and more consistent condition for solid phase synthesis and
therefore better quality of the bio-molecule synthesized.
1. Good mechanical stability for practical loading, easy to handle versus hard to catch for PEG resin as fluffy
2. Homogeneous swellability in most polar organic solvents.
3. Robust chemical stability against strong acid and strong base.
4. Good swellability in water or aqueous buffers. This is advantageous as chemical manipulation on a bio-
molecule can be feasible in aqueous system while the bio-molecule is still attached on the support, and
additionally, biochemical applications can be developed such as on-resin screening of chemical libraries
and affinity chromatography.
5. The resin is being proven useful for synthesis of bio-molecules with increasing number of peptides
successfully synthesized in high quality.
Comparison of Swellability
para-Hydroxymethylbenzamide is commonly known as HMBA linker. Li-Resin provides the p-benzyl alcohol
head group which is installed in a cross-linked acrylic amide polymer. P-Benzyl alcohol group is versatile
with a variety of reactive counterparts to form a covalent bond which can be cleaved by alkaline hydrolysis or
p-Oxybenzyl alcohol is commonly known as the head group of Wang-Resin. Li-Resin provides p-oxybenzyl
alcohol head group which is installed in a cross-linked acrylic amide polymer. P-Oxybenzyl alcohol group
is versatile with a variety of reactive counterparts to form a covalent bond which can be cleaved by alkaline
hydrolysis or ammonolytic detachment, or more usefully, by 90-95% TFA in dichloromethane in 1-2 hours to
give peptide product at the C-terminus with carboxylic acid.
Amino-Li-Resin—A Fiber Polyacrylamide Resin for Solid-Phase Peptide Synthesis (Article)