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작성일 : 22-05-10
[ImmuSmol] L-Asparagine ELISA

 

L-Asparagine ELISA

 

L-Asparagine is a conditionally non-essential amino acid that can be produced in our body. However,

Asparagine (Asn) is essential for the growth of highly proliferative cells such as cancers and it must be

provided by the environment. Targeting Asn using recombinant Asparaginase – as to deprive tumor cells of

an important energetic source – thus represents an attractive target and has been for instance approved for

the treatment of Acute Lymphoblastic Leukemia.

Also, while targeting Asn metabolism figures as an attractive therapeutic avenue, Asn level constitutes a

valuable biomarker. Indeed, among other evidences, it has been shown that quantification of Asn nicely

discriminates healthy subjects from patients with Epithelial Ovarian Cancer. More recently, increased Asn

level has been linked to poor survival in female patients with colorectal cancer.

 

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Cross-validation of L-Asparation ELISA and LC/MS data in human plasma samplesAsn was

quantified in human plasma samples from healthy subjects using IS-I-1600R ELISA kit or by LC/MS.

Correlation study showed R2=0.9774, thereby confirming the accuracy of the immunoassay.

 

Specifications

Format;

96-well plate

Sample;

Plasma  

Minimal sample volume;

20  µl

Species-reactivity;

React with all species

Standard range;

4.4 – 375µM (0.6 – 49.5µg/mL)

Sensitivity;

2.3µM (0.30µg/mL)

Specificity;

 

No significant cross-reactivity was observed with D-Asparagine, L-Aspartate, L-Glutamate, L-Glutamine.

Assay time;

Sample preparation 3h and ELISA overnight

Storage;

Store at 2 ~ 8°C for to 6 months.

 

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Typical standard curve of Arginine ELISA Typical standard curve obtained with the Asn ELISA kit

where the optical density is inversely correlated within levels within a linear range of 4.4 - 375μM. (example

data - do not use for calculation)

 

Instruction sheet (PDF)

   

References;

  1. Van Trimpont et al., Novel Insights on the Use of L-Asparaginase as an Efficient and Safe Anti-Cancer Therapy, Cancers, 2022

  2. Wang et al., Serum metabolite signatures of epithelial ovarian cancer based on targeted metabolomics, Clinica Chimica Acta, 2021

  3. Shen et al., Asparagine Metabolism in Tumors Is Linked to Poor Survival in Females with Colorectal Cancer: A Cohort Study, Metabolites, 2021

   



Ordering information

    

Catalog No.

Product Name

Size

IS-I-1600R

L-Asparagine ELISA kit

96 wells plate

 

 

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