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작성일 : 22-06-07
[Profoldin] Zika Virus RNA-dependent RNA Polymerase Assay
Viral (Flavivirus) RNA-dependent RNA Polymerase Assay Kits

 

Zika Virus RNA-dependent RNA Polymerase Assay/

Viral (Flavivirus) RNA-dependent RNA Polymerase Assay Kits

    

Zika virus is a positive, single-stranded, enveloped RNA virus. It is one of the Flaviviridae family. Zika virus

infection transmitted by mosquitoes is a trigger of Guillain-Barré syndrome, neuropathy and myelitis,

particularly in adults and older children. Infection in pregnancy is a cause of microcephaly and other

congenital abnormalities in the developing fetus and newborn. It also results in pregnancy complications

such as fetal loss, stillbirth, and preterm birth.

 

The RNA-dependent RNA Polymerase (RdRp) is an essential enzyme for the virus and is an attractive drug

target. The viral RdRp assay is based on detection of RNA synthesis by the RdRp enzyme.  The assay can

be performed in a 384-well or 96-well plate format for tests of the enzyme activity and high throughput

screening of Zika RdRp inhibitors.

 

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The Zika Virus RNA-dependent RNA Polymerase Assay Kit Plus-100 (Catalog No. ZRPA100KE)includes

350 µl of 10 x Buffer, 350 µl of 10 x MnCl2,  33 µl of 100 x Template, 33 µl of 100 x NTP, 31 µl of 100 x Zika

virus RdRp and 330 µl of 10 x fluorescence dye. It is for 100 assays of virus RNA polymerase in a 384-well

plate format.

 

The Viral (Flavivirus) RNA-dependent RNA Polymerase Assay Kit – 100 (Catalog No.VRT100K)includes

350 µl of 10 x Buffer, 350 µl of 10 x MnCl2,  33 µl of 100 x Template, 33 µl of 100 x NTP and 330 µl of 10 x

fluorescence dye. It is for 100 assays of virus RNA polymerase in a 384-well plate format. The assay kit

includes all reagents except the enzyme.   

 

The Viral (Flavivirus) RNA-dependent RNA Polymerase Assay Kit – 500 (Catalog No.VRT500K)includes

1550 µl of 10 x Buffer, 1550 µl of 10 x MnCl2,  160 µl of 100 x Template, 160 µl of 100 x NTPs and 1550 µl of 10

x fluorescence dye. It is for 500 assays of virus RNA polymerase in a 384-well plate format. The assay kit

includes all reagents except the enzyme.

 

 Instruction sheet (PDF)

 


Reference;

1, Shu Yang et al, Emetine inhibits Zika and Ebola virus infections through two molecular mechanisms:

   inhibiting viral replication and decreasing viral entry, Cell Discovery, Volume 4,  Article number: 31,

   (05 June 2018).

2. Chen Y. et al, Cinnamic acid inhibits Zika virus by inhibiting RdRp activity. Antiviral Research Volume 192,

   August 2021, 105117.

 

  

Ordering informations

   

Catalog No.

Product Name

Size

ZRPA100KE

Zika Virus RNA-dependent RNA Polymerase Assay Kit Plus-100

100 assays

VRT100K

Viral (Flavivirus) RNA-dependent RNA Polymerase Assay Kit 100

100 assays

VRT500K

Viral (Flavivirus) RNA-dependent RNA Polymerase Assay Kit-500

500 assays

 

 

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