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작성일 : 22-07-20
[HansaBioMed Life Sciences] TFF-EVs, Tangential flow filter for EV purification

 

TFF-EVs, Tangential flow filter for EV purification

Efficient and fast purification of EVs and nanoparticles

 

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Tangential flow filtration (TFF) is emerging as one of the most efficient methods for the purification of

extracellular vesicles for. TFF-EVs allows a rapid, reproducible and scalable purification of EVs, can be

used on the lab bench for purifying small amount of samples (min 5ml) or connected with a mechanical

system for purifying larger volumes.

 

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Speicifications;

• Hollow fiber filter pores: 50 nm

• Suitable for purification of small EVs and large EVs.

• Suitable for manual and mechanical use

 

TFF-EVs: bench purification workflow (manual use)

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Fast purification with minimal loss of particles

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1x1012particles of purified EVs (HBM PEU-100) were diluted in

20 ml of PBS 1x and then injected into TFF-EVs. Retentate

containing EVs was recovered in 5 ml of PBS 1x. The particle

content of the filtrate and retentate were analyzed by NTA

(Zetaview, Particle Metrix).

 

Depletion of bovine EVs from Fetal Bovine Serum (FBS)

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TFF-EVs was used to depleat the FBS from EVs of bovine origine. 50 ml of raw FBS were filtered through

TFF-EVs, the filtrate contained the deplated FBS, whereas bovine EVs were recovered from the retentate in

10 ml PBS 1x buffer. All the three fractions were analyzed by NTA ((Zetaview, Particle Metrix). EV depleted

FBS contains only the 1% of the total particles detected in the raw FBS.

 

Applications

• Easy washable

• Reusable multiple times

• Sterile

 

 

Ordering information;

   

Catalog No.

Product Name

Size

HBM-TFF/EVs

Tangential flow filtration (TFF) for EV purification

Small/Large

 

 

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