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Human Umbilical Vein Endothelial Cells

HUVECs (cAP-0001) are pooled cells isolated from normal human umbilical vein. The cells are shipped in frozen vials (the cells are provided @ passage 1). Endothelial Growth Medium (EGM, contains 10% serum and growth supplements, cAP-02) is recommended for cell culture and these cells have a minimum average population doubling levels > 20 when cultured following the detailed protocol described below).

Data sheet (PDF) 

GFP Expressing Human Umbilical Vein Endothelial Cells

HUVECs (cAP-0001) were infected with GFP- expressing Lentiviral particles @ passage 1. Puromycin resistant GFP-HUVECs (cAP-0001GFP) were selected and shipped in frozen vials (cells are provided @ passage 3). Endothelial Growth Medium (cAP-02) is recommended for cell culture and these cells have a minimum of 18 population doubling levels when cultured following the protocols described below).

Data sheet (PDF)  

RFP Expressing Human Umbilical Vein Endothelial Cells

HUVECs (cAP-0001) were infected with RFP- expressing Lentiviral particles @ passage 1. Zeocin resistant RPF-HUVECs (cAP-0001RFP) were selected and shipped in frozen vials (cells are provided @ passage 3). Endothelial Growth Medium (cAP-02) is recommended for cell culture and these cells have a minimum of 18 population doubling levels when cultured following the protocols described below).

Data sheet (PDF)  

Characterization of the cells 

1. Cytoplasmic VWF/Factor VIII >95% positive by immunofluorescence

2. Cytoplasmic uptake of Di-I-Ac-LDL >95% positive by immunofluorescence

3. Cytoplasmic PECAM1 >95% positive by immunofluorescence

4. HUVECs are negative for HIV-1, HBV, HCV, and mycoplasma

Product Format:            Frozen Vial

Cell Number:           > 5x10[5] cells/vial

Shipping:                   Frozen Vials in Dry Ice package.

Handling of arriving cells: When you receive the dry ice package with cells in frozen vials, transfer the frozen vials of cells into a -80C freezer for short period storage or 

                              a liquid nitrogen tank for long- term storage.

▣ 관련 페이지 ; Angio-Proteomie