| Apoptotic DNA Ladder kit     The Apoptotic DNA Ladder kit contains all of the reagents necessary for the preparation of cellular fractions  for the production of apoptosis nucleosomal DNA ladders. The kit does not require the use of toxic phenol.  The protocol involves: cell lysis; removal of cellular debris; and precipitation of nucleosomal DNA.  Nucleosomal DNA is detected after standard 1.8% agarose gel electrophoresis. The protocol providesthe  choice of preparing nucleosomal DNA ladder either with or without genomic DNA. The kit is suitable for  preparing up to 100 apoptotic ladders.     Instruction sheet (PDF)     References Rajathi K., et al (2017) IN VITRO CYTOTOXICITY TESTING OF BIOSYNTHESISED SILVER NANOPARTICLE OF ANDREDERA CORDIFOLIA IN PROSTATE CANCER CELLS (PC-3) ejpmr 4(1):401Wani, W.Y. et al (2016) Cell cycle activation in p21 dependent pathway: An alternative mechanism of organophosphate induced dopaminergic neurodegeneration.Biochim. Biophys. Acta.doi:10.1016/j.bbadis.2016.05.014Krishna, S.G. et al (2015) Cytotoxicity of chloroform extract of Toddalia Asiatica L. Leaf.  Int. J. Ayur. Pharma Research, 2015;3(6):40-44.Krishna, S.G. (2015) IJRPB. 3:210Krishna, S.G. et al (2015) Int. J. Ayur. Pharma Research 3:40Kulkarni, A et al (2015) IAJPR. 5(2): 937Al-Fatlawi, A.A. et al. (2014) World J Pharm Sci 2: 1218Al-Ftlawi, A. et al (2014) Int J Pharm Pharm Sci. 6:515Kireev, R. et al (2013) Eur. J. Pharmacol. 701:185Sunkaria, A. et al (2012) Chem. Res. Toxicol. 25:1762Qa'Dan, M. et al (2002) Cell. Microbiol. 4:425
     Instruction sheet (pdf)    |