|
Atto-Actin (alpha actin, rabbit skeletal muscle)_II
The Colours of Actin!
The bright way to visualize actin interactions...
Atto-Actins are important tools for investigating actin dynamics.
Ready-to-use fluorescent actin conjugates suited for numerous state-of-the-art techniques. Atto-actins are
used for all types of fluorescence microscopes: from epifluorescence to high resolution laser microscopy,
biophysical spectroscipcal methods and biochemical applications. The novel Atto-Actins are characterized
by high photo stabilty, strong absorption and high fluorescence quantum yields - some of several features
demanded for todays imaging techniques.
These fluorescent actin conjugates are prepared by esterification of lysine residues with activated Atto dyes.
Atto-Actins are validated to possess the polymerization properties of native actin.
Atto-Actins are worldwide exclusively manufactured by Hypermol.
Applications
TIRF Microscopy, FRET, Confocal Microscopy, Epifluorescence Microscopy, Biophysical Spectroscopical
Techniques, Biochemical Actin Interaction Assays...
Atto532-Actin (alpha actin skeletal muscle, rabbit)
|
|
ATTO 532
• High fluorescence yield
• High photostability & small Stokes-shift
• Especially suited for Argon-Ion & Nd: YAG laser
|
Description
|
Protein
|
|
Atto532-Actin
|
|
Origin
|
|
skeletal muscle alpha actin, rabbit
|
|
Molecular mass
|
|
42kDa
|
|
Protein description
|
|
Actin from rabbit skeletal muscle is a single chain polypeptide with a molecular
weight of 42kD consisting of 375 amino acids. Atto532-Actin is chemically modified
G-actin with an Atto532 fluorescent group covalently bound to lysine residues.
Atto532-Actin (λem 532nm, λem 553nm) possesses the polymerization properties
of native G-actin.
|
|
Conjugation ratio
|
|
This product is supplied with a degree of labelling (DOL) between 0.5 and 0.8.
The precise DOL is stated in the DataSheet supplied with the product.
|
Properties
|
Form
|
|
Lyophilized, ready-to-use
|
|
Quantity
|
|
1x100 µg, 5x100 µg
|
|
Buffer
|
|
2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3%
disaccharides, when reconstituted with 1.0 ml ultrapure water to obtain a 1mg/ml
solution.
|
|
Purity
|
|
>99% by scanning densitometry
|
|
Purification notes
|
|
Purified from rabbit skeletal muscle.
|
|
Protein conc.
|
|
Determined by the Biuret method.
|
|
Storage instructions
|
|
Atto532-Actins are stored at -70°C upon arrival will be stable in performance for
at least 6 months from the date of purchase. Solubilized Atto-actin is kept on ice
and should be used within 1 week. Avoid refreezing.
|
|
Shipping conditions
|
|
At ambient temperature. Upon delivery store at -70°C.
|
|
Remarks
|
|
For Use in Research only. Not for Use in Human or Veterinary Diagnostical or
Therapeutical Applications.
|
Atto550-Actin (alpha skeletal muscle actin, rabbit)
|
|
ATTO 550
• High fluorescence yield
• Strong absorption, high photo-stability
• High resolution microscopy, single molecule detection
|
Description
|
Protein
|
|
Atto550-Actin
|
|
Origin
|
|
skeletal muscle alpha actin, rabbit
|
|
Molecular mass
|
|
42kDa
|
|
Protein description
|
|
Actin from rabbit skeletal muscle is a single chain polypeptide with a molecular
weight of 42kD consisting of 375 amino acids. Atto550-Actin is chemically modified
G-actin with an Atto565 fluorescent group covalently bound to lysine residues.
Atto550-Actin (λem 550nm, λem 576nm) possesses the polymerization properties
of native G-actin.
|
|
Conjugation ratio
|
|
This product is supplied with a degree of labelling (DOL) between 0.5 and 0.8.
The precise DOL is stated in the DataSheet supplied with the product.
|
Properties
|
Form
|
|
Lyophilized, ready-to-use
|
|
Quantity
|
|
1x100 µg, 5x100 µg
|
|
Buffer
|
|
2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3%
disaccharides, when reconstituted with 1.0 ml ultrapure water to obtain a 1mg/ml
solution.
|
|
Purity
|
|
>99% by scanning densitometry
|
|
Purification notes
|
|
Purified from rabbit skeletal muscle, GPC.
|
|
Protein conc.
|
|
Determined by the Biuret method.
|
|
Storage instructions
|
|
Atto550-Actins are stored at -70°C upon arrival will be stable in performance for
at least 6 months from the date of purchase. Solubilized Atto-Actin is kept on
ice and should be used within 1 week. Avoid refreezing
|
|
Shipping conditions
|
|
At ambient temperature. Upon delivery store at -70°C.
|
|
Remarks
|
|
For Use in Research only. Not for Use in Human or Veterinary Diagnostical or
Therapeutical Applications.
|
Atto594-Actin (alpha skeletal muscle actin, rabbit)
Description
|
Protein
|
|
Atto594-Actin
|
|
Origin
|
|
skeletal muscle alpha actin, rabbit
|
|
Molecular mass
|
|
42kDa
|
|
Protein description
|
|
Actin from rabbit skeletal muscle is a single chain polypeptide with a molecular
weight of 42kD consisting of 375 amino acids. Atto594-Actin is chemically modified
G-actin with an Atto594 fluorescent group covalently bound to lysine residues.
Atto594-Actin (λem 601nm, λem 627nm) possesses the polymerization properties
of native G-actin.
|
|
Conjugation ratio
|
|
This product is supplied with a degree of labelling (DOL) between 0.5 and 0.8.
The precise DOL is stated in the DataSheet supplied with the product.
|
Properties
|
Form
|
|
Lyophilized, ready-to-use
|
|
Quantity
|
|
1x100 µg, 5x100 µg
|
|
Buffer
|
|
2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3%
disaccharides, when reconstituted with 1.0 ml ultrapure water to obtain a 1mg/ml
solution.
|
|
Purity
|
|
>99% by scanning densitometry
|
|
Purification notes
|
|
Purified from rabbit skeletal muscle.
|
|
Protein conc.
|
|
Determined by the Biuret method.
|
|
Storage instructions
|
|
Atto594-Actin are stored at -70°C upon arrival will be stable in performance for
at least 6 months from the date of purchase. Solubilized Atto-actin is kept on ice
and should be used within 1 week. Avoid refreezing of actin.
|
|
Shipping conditions
|
|
At ambient temperature. Upon delivery store at -70°C.
|
|
Remarks
|
|
For Use in Research only. Not for Use in Human or Veterinary Diagnostical or
Therapeutical Applications.
|
Atto647-Actin (alpha skeletal muscle actin, rabbit)
Description
|
Protein
|
|
Atto647-Actin
|
|
Origin
|
|
skeletal muscle alpha actin, rabbit
|
|
Molecular mass
|
|
42kDa
|
|
Protein description
|
|
Actin from rabbit skeletal muscle is a single chain polypeptide with a molecular
weight of 42kD consisting of 375 amino acids. Atto647-Actin is chemically modified
G-actin with an Atto647 fluorescent group covalently bound to lysine residues.
Atto647-Actin (λem 647nm, λem 669nm) possesses the polymerization properties
of native G-actin.
|
|
Conjugation ratio
|
|
This product is supplied with a degree of labelling (DOL) between 0.5 and 0.8.
The precise DOL is stated in the DataSheet supplied with the product.
|
Properties
|
Form
|
|
Lyophilized, ready-to-use
|
|
Quantity
|
|
1x100 µg, 5x100 µg
|
|
Buffer
|
|
2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3%
disaccharides, when reconstituted with 1.0 ml ultrapure water to obtain a 1mg/ml
solution.
|
|
Purity
|
|
>99% by scanning densitometry
|
|
Purification notes
|
|
Purified from rabbit skeletal muscle.
|
|
Protein conc.
|
|
Determined by the Biuret method.
|
|
Storage instructions
|
|
Atto647-Actins are stored at -70°C upon arrival will be stable in performance for
at least 6 months from the date of purchase. Solubilized Atto-actin is kept on ice
and should be used within 1 week. Avoid refreezing of actin.
|
|
Shipping conditions
|
|
At ambient temperature. Upon delivery store at -70°C.
|
|
Remarks
|
|
For Use in Research only. Not for Use in Human or Veterinary Diagnostical or
Therapeutical Applications.
|
|