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Atto-Actin (alpha cardiac actin, bovine cardiac muscle)
The Colours of Actin!
The bright way to visualize actin interactions.
Atto-Actins are important tools for investigating actin dynamics.
Ready-to-use fluorescent actin conjugates suited for numerous state-of-the-art techniques. Atto-actins are
used for all types of fluorescence microscopes: from epifluorescence to high resolution laser microscopy,
biophysical spectroscipcal methods and biochemical applications. The novel Atto-Actins are characterized
by high photo stabilty, strong absorption and high fluorescence quantum yields - some of several features
demanded for todays imaging techniques.
These fluorescent actin conjugates are prepared by esterification of lysine residues with activated Atto dyes.
Atto-Actins are validated to possess the polymerization properties of native actin.
Atto-Actins are worldwide exclusively manufactured by Hypermol.
Applications
TIRF Microscopy, FRET, Confocal Microscopy, Epifluorescence Microscopy, Biophysical Spectroscopical
Techniques, Biochemical Actin Interaction Assays.
Atto390-Actin (alpha-cardiac actin, bovine)
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ATTO 390
• High fluorescence yield
• Large Stokes-shift
• Wel suited for all types of fluorescence microscopes
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Description
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Protein
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Atto390-Actin
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Origin
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Alpha cardiac actin, rabbit
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Molecular mass
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42kDa
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Protein description
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Actin from bovine cardiacl muscle is a single chain polypeptide with a molecular
weight of 42kD consisting of 375 amino acids. Atto390-Actin is chemically modified
G-actin with an Atto390 fluorescent group covalently bound to lysine residues.
Atto390-Actin (λem 390nm, λem 479nm) possesses the polymerization properties
of native G-actin.
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Conjugation ratio
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This product is supplied with a degree of labelling (DOL) between 0.5 and 0.8.
The precise DOL is stated in the DataSheet supplied with the product.
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Properties
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Form
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Lyophilized, ready-to-use
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Quantity
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1x100 µg, 5x100 µg
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Buffer
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2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3%
disaccharides, when reconstituted with 1.0 ml ultrapure water to obtain a 1mg/ml
solution.
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Purity
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>99% by scanning densitometry
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Purification notes
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Purified from bovine cardiac muscle.
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Protein conc.
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Determined by the Biuret method.
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Storage instructions
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Atto-Actins are stored at -70°C upon arrival will be stable in performance for
at least 6 months from the date of purchase. Solubilized Atto-actin is kept on ice
and should be used within 1 week. Avoid refreezing.
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Shipping conditions
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At ambient temperature. Upon delivery store at -70°C.
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Atto532-Actin (alpha-cardiac actin, bovine)
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ATTO 532
• High fluorescence yield
• High photostability & small Stokes-shift
• Especially suited for Argon-Ion & Nd: YAG laser
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Description
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Protein
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Atto532-Actin
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Origin
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Alpha cardiac actin, bovine
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Molecular mass
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42kDa
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Protein description
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Actin from bovine cardiac muscle is a single chain polypeptide with a molecular
weight of 42kD consisting of 375 amino acids. Atto532-Actin is chemically modified
G-actin with an Atto532 fluorescent group covalently bound to lysine residues.
Atto532-Actin (λem 532nm, λem 553nm) possesses the polymerization properties
of native G-actin.
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Conjugation ratio
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This product is supplied with a degree of labelling (DOL) between 0.5 and 0.8.
The precise DOL is stated in the DataSheet supplied with the product.
|
Properties
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Form
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|
Lyophilized, ready-to-use
|
|
Quantity
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1x100 µg, 5x100 µg
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|
Buffer
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2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3%
disaccharides, when reconstituted with 1.0 ml ultrapure water to obtain a 1mg/ml
solution.
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Purity
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>99% by scanning densitometry
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Purification notes
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Purified from bovine cardiac muscle.
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Protein conc.
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Determined by the Biuret method.
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Storage instructions
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Atto-Actins are stored at -70°C upon arrival will be stable in performance for
at least 6 months from the date of purchase. Solubilized Atto-Actin is kept on
ice and should be used within 1 week. Avoid refreezing.
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Shipping conditions
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At ambient temperature. Upon delivery store at -70°C.
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