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Atto-Actin for TIRFM
Fluorescent Actin-Conjugates
Ready-for-TIRFM
Highly photostable Atto-Actins are ideal to visualize actin dynamics by TIRFM.
Atto-fluorescent actin conjugates for TIRFM are labelled with a DOL of 0.3 and thus ready-for TIRFM.
The novel Atto-Actins are characterized by high photo stability, strong absoprtion and high fluorescence
quantum yields - some of several features demanded for todays imaging techniques.
These fluorescent actin conjugates are prepared by esterification of lysine residues with activated
Atto dyes. Atto-Actins are validated to possess the polymerization properties of native actin.
Atto-Actins are worldwide exclusively manufactured by Hypermol.
Applications
TIRFM, STED, GSD.
Atto565-Actin for TIRFM (alpha skeletal muscle actin, rabbit)
Description
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Protein
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Atto565-Actin
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Origin
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skeletal muscle alpha actin, rabbit
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Molecular mass
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42kDa
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Protein description
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Actin from rabbit skeletal muscle is a single chain polypeptide with a molecular
weight of 42kD consisting of 375 amino acids. Atto565-Actin is chemically modified
G-actin with an Atto565 fluorescent group covalently bound to lysine residues.
Atto565-Actin (λem 563nm, λem 592nm) possesses the polymerization properties
of native G-actin.
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Conjugation ratio
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This product is supplied with a degree of labelling (DOL) between 0.3
The precise DOL is stated in the DataSheet supplied with the product.
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Properties
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Form
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Lyophilized, ready-to-use
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Quantity
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1x100 µg, 5x100 µg
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Buffer
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2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3%
disaccharides, when reconstituted with 1.0 ml ultrapure water to obtain a 1mg/ml
solution.
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Purity
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>99% by scanning densitometry
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Purification notes
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Purified from rabbit skeletal muscle.
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Protein conc.
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Determined by the Biuret method.
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Storage instructions
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Atto565-Actins are stored at -70°C upon arrival will be stable in performance for
at least 6 months from the date of purchase. The Solubilized product is kept on ice,
protect from light and it should be used within 1 week. Avoid refreezing.
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Shipping conditions
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At ambient temperature. Upon delivery store at -70°C.
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Remarks
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For Use in Research only. Not for Use in Human or Veterinary Diagnostical or
Therapeutical Applications.
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Atto488-Actin for TIRFM (alpha skeletal muscle actin, rabbit)
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ATTO 488
• High fluorescence yield
• Strong absorption & high photo-stability
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Description
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Protein
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Atto488-Actin
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Origin
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skeletal muscle alpha actin, rabbit
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Molecular mass
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42kDa
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Protein description
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Actin from rabbit skeletal muscle is a single chain polypeptide with a molecular
weight of 42kD consisting of 375 amino acids. Atto488-Actin is chemically modified
G-actin with an Atto488 fluorescent group covalently bound to lysine residues.
Atto488-Actin (λem 488nm, λem 523nm) possesses the polymerization properties
of native G-actin.
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Conjugation ratio
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This product is supplied with a degree of labelling (DOL) of ~0. 3.
The precise DOL is stated in the DataSheet supplied with the product.
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Properties
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Form
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|
Lyophilized, ready-to-use
|
|
Quantity
|
|
1x100 µg, 5x100 µg
|
|
Buffer
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2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3%
disaccharides, when reconstituted with 1.0 ml ultrapure water to obtain a 1mg/ml
solution.
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Purity
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>99% by scanning densitometry
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Purification notes
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Purified from rabbit skeletal muscle, GPC.
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Protein conc.
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Determined by the Biuret method.
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Storage instructions
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Atto565-Actins are stored at -70°C upon arrival will be stable in performance for
at least 6 months from the date of purchase. Solubilized Atto488-Actin is kept
on ice, protected from light and should be used within 1 week. Avoid refreezing.
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Shipping conditions
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At ambient temperature. Upon delivery store at -70°C.
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Remarks
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For Use in Research only. Not for Use in Human or Veterinary Diagnostical or
Therapeutical Applications.
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Atto550-Actin for TIRFM (alpha skeletal muscle actin, rabbit)
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ATTO 550
• High fluorescence yield
• Strong absorption, high photo-stability
• High resolution microscopy, single molecule detection
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Description
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Protein
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Atto550-Actin
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Origin
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skeletal muscle alpha actin, rabbit
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Molecular mass
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42kDa
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Protein description
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Actin from rabbit skeletal muscle is a single chain polypeptide with a molecular
weight of 42kD consisting of 375 amino acids. Atto550-Actin is chemically modified
G-actin with an Atto550 fluorescent group covalently bound to lysine residues.
Atto550-Actin (λem 550nm, λem 576nm) possesses the polymerization properties
of native G-actin.
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Conjugation ratio
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This product is supplied with a degree of labelling (DOL) between 0.5 and 0.8.
The precise DOL is stated in the DataSheet supplied with the product.
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Properties
|
Form
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|
Lyophilized, ready-to-use
|
|
Quantity
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|
1x100 µg, 5x100 µg
|
|
Buffer
|
|
2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3%
disaccharides, when reconstituted with 1.0 ml ultrapure water to obtain a 1mg/ml
solution.
|
|
Purity
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>99% by scanning densitometry
|
|
Purification notes
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Purified from rabbit skeletal muscle.
|
|
Protein conc.
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Determined by the Biuret method.
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Storage instructions
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Atto550-Actin are stored at -70°C upon arrival will be stable in performance for
at least 6 months from the date of purchase. Solubilized Atto-actin is kept on
ice and should be used within 1 week. Avoid refreezing.
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|
Shipping conditions
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At ambient temperature. Upon delivery store at -70°C.
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Remarks
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For Use in Research only. Not for Use in Human or Veterinary Diagnostical or
Therapeutical Applications.
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Atto647-Actin for TIRFM (alpha skeletal muscle actin, rabbit)
Description
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Protein
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Atto647-Actin
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Origin
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skeletal muscle alpha actin, rabbit
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Molecular mass
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42kDa
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Protein description
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|
Actin from rabbit skeletal muscle is a single chain polypeptide with a molecular
weight of 42kD consisting of 375 amino acids. Atto647-Actin is chemically modified
G-actin with an Atto647 fluorescent group covalently bound to lysine residues.
Atto647-Actin (λem 647nm, λem 669nm) possesses the polymerization properties
of native G-actin.
|
|
Conjugation ratio
|
|
This product is supplied with a degree of labelling (DOL) between 0.3
The precise DOL is stated in the DataSheet supplied with the product.
|
Properties
|
Form
|
|
Lyophilized, ready-to-use
|
|
Quantity
|
|
1x100 µg, 5x100 µg
|
|
Buffer
|
|
2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3%
disaccharides, when reconstituted with 1.0 ml ultrapure water to obtain a 1mg/ml
solution.
|
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Purity
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>99% by scanning densitometry
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|
Purification notes
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Purified from rabbit skeletal muscle.
|
|
Protein conc.
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Determined by the Biuret method.
|
|
Storage instructions
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|
Atto647-Actins are stored at -70°C upon arrival will be stable in performance for
at least 6 months from the date of purchase. The Solubilized product is kept on ice,
protect from light and should be used within 1 week. Avoid refreezing of actin.
|
|
Shipping conditions
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At ambient temperature. Upon delivery store at -70°C.
|
|
Remarks
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|
For Use in Research only. Not for Use in Human or Veterinary Diagnostical or
Therapeutical Applications.
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