Products

EndotoxinOUT™, endotoxin removable resin 및 spin columns

For the rapid removal of endotoxins/pyrogens (LPS, lipopolysaccharides) from samples.

EndotoxinOUT™ resin은 polymyxin B를 agarose 담체에 고정화 한 것으로,  용액 중의 endotoxin과 LPS

(Lipopolysaccharides) 등과 결합하여 제거 할 수 있습니다. Polymyxin B는 polymyxin B1과 B2를 주성분으로 하는

항생물질로, 세균성 LPS의 lipid A 음이온 부위에 결합합니다.

제품에는 spin column에 resin을 충전해서 사용하는 것과 편리하게 사용할 수 있도록 일회용 column에 충전되어

있는 2가지가 있습니다.

EndotoxinOUT™ was designed for the rapid removal of endotoxins and pyrogens (LPS, lipopolysaccharides) from

nucleic acid (DNA) samples. EndotoxinOUT™ is an ideal product for the cleanup of buffers, cell culture media,

protein solutions, nucleic acid (DNA) samples and pharmacological components.

EndotoxinOUT™ consists of 6% cross-linked agarose covalently linked to polymyxin B to bind and remove harmful

pyrogens from a solution. Polymyxin B is a family, polymyxin B1 and B2, of antibiotics that bind to the negatively

charged site of the lipid A portion of the bacterial lipopolysaccharide layer, neutralizing the endotoxic activity. The

covalently coupled agarose and polymyxin B is a stable matrix that resists leaching.

Features

• Polymyxin B Sulfate immobilized on 6% cross-linked agarose

• Capacity: ≥9995 endotoxin units (EU) removed by 1ml resin from 5ml test containing 10,000EU

• Fractionation Range: 10‐4,000kDa for proteins

• Minimal leaching

• Reusable at least 10 times

Applications

• Clean up of buffers, cell culture media, protein solutions, DNA samples & pharmacological components

• Rapid 99.95% removal of endotoxins & pyrogens from samples

Important information

• Non specific binding of hydrophobic molecules may occur. To limit non‐specific binding buffer all solutions to

  physiological pH. To inhibit ionic interactions, use a final concentration of 0.1‐0.5M NaCl.

• Avoid the use of chaotropes and detergents as this interfere with binding to polymyxin sulfate.

• Some proteins, including BSA, bind tightly to endotoxins reducing their interaction and removal by the

  polymyxin sulfates. Increasing the volume of EndotoxinOUT™ resin to endotoxin containing sample may

  help.

• In some cases, the interaction between endotoxin and polymyxin sulfate is not inhibited, resulting in

  immobilization of the protein to the resin. Page 2 of 4

• Optimal performance of the EndotoxinOUT™ is best achieved under gravity flow chromatography, with an

  incubation period, allowing for more time for endotoxins to be in contact with polymyxin sulfate.

Instruction sheet (PDF)

Catalog No.

Product Name

Size

786-367

EndotoxinOUT™

10ml resin

786-368

1L resin

786-369

 5 x 1ml columns