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Anti-Mouse IgG F(ab')2 Atto

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Anti-Mouse IgG F(ab')2 Atto

Highly specific secondary antibodies - strong & long lasting fluorescence!

매우 안정한 dyes에 conjugate한 항체로, 매우 높은 affinity를 갖고 있습니다. 이미 독일과 유럽의 연구자들

사이에 이미 정평이 있으면서도 가격은 상대적으로 저렴합니다. 이러한 제품을 보다 널리 보급하기 위하여

8월 말까지 10% 할인된 가격으로 제공합니다.

The quality of secondary antibodies is essential in producing superior results in immunofluorescence.

Hypermol offers secondary antibodies conjugated to the novel, high quality Atto dyes for detecting primary

antibodies. Atto-labeled antibodies belong to the next generation fluorescent antibodies, characterized by

their exceptional fluorescence intensity and superior photostability. For this reason they are suited for all

kinds of immunofluorescence microscopy and especially for high and super-resolution techniques.

Atto-conjugated F(ab')2 fragments are affinity purified IgGs cleaved by pepsin to remove the Fc part to avoid

interaction with Fc-receptors. Further purification by Size Exclusion Chromatography (SEC) yield highly pure

F(ab')2. The antibodies and fragments thereof are quality-control tested by UV-VIS spectroscopy and

immunocytochemistry. They come in a safety box with a detailed product documentation.

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Anti-Mouse IgG F(ab')2-Atto488, Antibody produced in goat

Description

Product name		Anti-mouse IgG F(ab')2-Atto488
Target species		Mouse
Description		Atto-labeled F(ab') fragment belong to the new generation of fluorescent antibodies characterized by their exceptional fluorescence intensity and superior photostability. Anti-Mouse IgG F(ab')2-Atto488 is specifically suited for high and super resolution microscopy, due to the high photostability and strong fluorescence quantum yield. Conjugates of F(ab')2 fragments are preferable to whole antibody conjugates, since they do not interact with Fc receptor-bearing membranes, due to the absence of the antibody Fc region.
Specifications		Goat anti-mouse IgG F(ab')2 Atto488 (H+L), Immunofluorescence: 1:300-1:000, Degree of labeling (DOL) : >2-5, Unconjugated dye ≤5% of total fluorescence. For further information please see DataSheet.
Optical properties		λex 501nm, λem 523nm

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Properties

Form		Lyophilized
Concentration		2mg/ml
Content		0.5mg Anti-Mouse IgG F(ab') Atto488* Product documentation
Buffer		50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, 5 mM sodium azide, pH 7.4.
Clonality		Polyclonal
Isotype		IgG
Purification notes		Affinity purified IgG, SEC purified after pepsin cleavage.
Storage condition		Store as glycerol stock at -20°C. Avoid repeated freeze / thaw cycles.
Shipping condition		Shipped at ambient temperature

Anti-Mouse IgG F(ab')2-Atto565, Antibody produced in goat

Description

Product name		Anti-Mouse IgG F(ab')2-Atto565
Target species		Mouse
Description		Atto-labeled F(ab')2 fragments belong to the new generation fluorescent antibodies characterized by their exceptional fluorescence intensity and superior photostability. Anti-Mouse IgG F(ab')2-Atto565 is specifically suited for high resolution microscopy, due to the excellent photostability and strong fluorescence quantum yield. Conjugates of F(ab')2 fragments are preferable to whole antibody conjugates, sincethey do not interact with Fc receptor-bearing membranes, due to the absence of the antibody Fc region.
Specifications		Goat anti-mouse igG F(ab')2-Atto565 (H+L), Immunofluorescence: 1:300-1:1000, Degree of labeling (DOL) : >2-5, Unconjugated dye ≤5% of total fluorescence. For further information please see DataSheet.
Optical properties		λex 563nm, λem 592nm

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Properties

Form		Lyophilized
Concentration		2mg/ml
Content		0.5mg (2mg/ml) Anti-Mouse IgG F(ab')2-Atto565* 1.0ml Glycerol buffer Product documentation
Buffer		50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, 5 mM sodium azide, pH 7.4.
Clonality		Polyclonal
Isotype		IgG
Purification notes		Affinity purified IgG, SEC purified after pepsin cleavage.
Storage condition		Store as glycerol stock at -20°C. Avoid repeated freeze / thaw cycles.
Shipping condition		Ambient temperature.

Anti-Mouse IgG F(ab')2-Atto594, Antibody produced in goat

Description

Product name		Goat anti-mouse IgG F(ab')2-Atto594
Target species		Mouse
Description		Atto-labeled secondary antibodies F(ab')2 fragments belong to the new generation of fluorescent antibodies, characterized by their exceptional fluorescence intensity and superior photostability. Anti-Mouse IgG F(ab')2 Atto594 is specifically suited for high resolution microscopy, due to the high photostability and strong fluorescence quantum yield. Conjugates of F(ab')2 fragments are preferable to whole antibody conjugates, since they do not interact with Fc receptor-bearing membranes, due to the absence of the antibody Fc region.
Specifications		Goat anti-mouse IgG F(ab')2 Atto594 (H+L), Immunofluorescence: 1:300-1:1000, Degree of labeling (DOL) : >2-5, Unconjugated dye ≤5% of total fluorescence. For further information please see DataSheet.
Optical properties		λex 601nm, λem 627nm

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Properties

Form		Lyophilized
Concentration		2mg/ml
Content		0.5mg (2mg/ml) Anti-Mouse IgG F(ab')2-Atto594* 1.0ml Glycerol buffer Product documentation
Buffer		50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, 5 mM sodium azide, pH 7.4.
Clonality		Polyclonal
Isotype		IgG
Purification notes		Affinity purified IgG, SEC purified after pepsin cleavage.
Storage condition		Store as glycerol stock at -20°C. Avoid repeated freeze / thaw cycles.
Shipping condition		Ambient temperature

Table of dyes and light sources

Secondary Antibodies series

Anti-Mouse IgG Atto

Anti-Mouse IgG F(ab) Atto

Anti-Mouse IgG F(ab')2 Atto

Anti-Mouse IgM Atto

Anti-Rabbit IgG Atto

Anti-Rabbit IgG F(ab) Atto

Ordering informations

Catalog No.	Product Name	Size
2402-0.5MG (PDF)	Anti-Mouse IgG F(ab')2-Atto488 (H+L)	kit
2407-0.5MG (PDF)	Anti-Mouse IgG F(ab')2-Atto565 (H+L)	kit
2416-0.5MG (PDF)	Anti-Mouse IgG F(ab')2-Atto594 (H+L)	kit
2418-0.5MG (PDF)	Anti-Mouse IgG F(ab')2-Atto647N (H+L)	kit
2411-0.5MG (PDF)	Anti-Mouse IgG F(ab')2-Atto700 (H+L)	kit
2409-0.5MG (PDF)	Anti-Mouse IgG F(ab')2-Atto740 (H+L)	kit

Catalog of Atto secondary antibodies (PDF)

▣ 관련 페이지 ; HYPERMOL EK

이전글Anti-Mouse IgM Atto 14.06.25
다음글Anti-Mouse IgG F(ab) Atto, Atto-conjugated Fab-fragments 14.06.25

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