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Zyagen offers high quality pure intact total RNA of leaves of large variety of plant species. Total RNA is routinely

extracted from plant tissues usining classical liquid:liquid RNA extraction method and column purified. RNA is treated

with RNase-free DNase to remove residual DNA, precisely quantified, and stored at -80^oC. The integrity of each RNA

sample as indicated by intact ribosomal RNA is verified by denatured agarose gel electrophoresis. The purity of

RNA is assessed by spectrophotometer (A260/A280: 1.9-2.1). Residual DNA contamination is tested by PCR.

RNA is ideal for Northern blotting, ribonuclease protection assay, SI nuclease assay, RT-PCR/Q-PCR/RACE analysis,

cDNA synthesis, RNA differential display, microRNA studies, and purification of mRNA for library construction.

Total RNA sample is routinely provided in RNase-free water at a concentration of 1 mg/ml and shipped on dry ice.

Sample can also be provided in 1mM sodium citrate, 0.1 mM EDTA, or any other RNA storage buffer.

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