Products

Protein

Atto390-Actin (α-Actin)

Origin

Skeletal muscle alpha actin, rabbit

Molecular mass

42kDa

Toolkit description

The Actin-Toolkit Fluorescence Microscopy was developed to analyze and

demonstrate the effect of ligands on actin polymerization, or ligand-induced

formation of actin bundles and networks. In the assays_Atto390-Actin

(λem 390nm, λem 479nm) is mixed with the ligand to observe the effects on

either G- or F-actin. In contrast to staining with phallodin conjugates, which can

interfere with ligand binding sites on the actin molecule, no such observations

were made with Atto labeled actin. Atto-Actins are validated to possess the

polymerization properties of native actin.

The Toolkit further contains unlabeled actin (0.5mg α-skeletal muscle actin).

A mixture of both excludes possible interference of the fluorescent actin conjugates

on ligand binding sites. Vinculin tail - a V8 protease cleavage

product of vinculin - is and efficient actin bundling vinculin domain, is supplied

with the Toolkitas a bundling reference.

several standardized experiments are described step-by-step in the handbook

supplied with the kit. In combination with the actin buffers contained in the kit,

error-free handling is guaranteed. The kit contains 4x100µg of α-skeletal of

α-skeletal muscle Atto390-Actin conjugates and buffers to keep the actin functional.

Purity

 >99% by scanning densitometry

Form

Lyophilized, ready-to-use

Kit content

4x100µg Atto390-Actin (lyophilized powder)

1x0.5mg α-skeletal muscle actin (lyophilized powder)

1x50µg Vinculin tail

5x50ml MonoMix, (lyophilized G-actin ATP-buffer)

1x1.0ml PolyMix 10x stock (lyophilized F-actin ATP-buffer, pH 7.4)

1x0.5ml 1M MgCl2

1x Toolkit Handbook Fluorescence Microscopy

10x Light protective tubes & tags

Actin Buffer

2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3%

disaccharides, when reconstituted with 1.0 ml ultrapure water to obtain a 1mg/ml

solution.

Purification notes

Purified from rabbit skeletal muscle, GPC.

Protein concent.

Determined by the Biuret method

Storage instructions

We guarantee stable performance of the kit components for 6 months when stored at

-70°C upon arrival. Solubilized G-actin is kept on ice and should be used within 1

week. Avoid refreezing.

Shipping conditions

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or

Therapeutical Applications.

CAS no.

51005-14-2 (Actin)

Protein

Atto390-Actin (α-cardiac Actin)

Origin

Alpha-cardiac actin, bovine

Molecular mass

42kDa

Toolkit description

The Actin-Toolkit Fluorescence Microscopy was developed to analyze and

demonstrate the effect of ligands on actin polymerization, or ligand-induced

formation of actin bundles and networks. In the assays_Atto390-Actin

(λem 390nm, λem 479nm) is mixed with the ligand to observe the effects on

either G- or F-actin. In contrast to staining with phallodin conjugates, which can

interfere with ligand binding sites on the actin molecule, no such observations

were made with Atto labeled actin. Atto-Actins are validated to possess the

polymerization properties of native actin.

The Toolkit further contains unlabeled actin (0.5mg α-cardiac actin).

A mixture of both excludes possible interference of the fluorescent actin conjugates

on ligand binding sites. Vinculin tail - a V8 protease cleavage

product of vinculin - is and efficient actin bundling vinculin domain, is supplied

with the Toolkitas a bundling reference.

several standardized experiments are described step-by-step in the handbook

supplied with the kit. In combination with the actin buffers contained in the kit,

error-free handling is guaranteed. The kit contains 4x100µg of α-cardiac

Atto390-Actin conjugates and buffers to keep the actin functional.

Purity

 >99% by scanning densitometry

Form

Lyophilized, ready-to-use

Kit content

4x100µg Atto390-Actin (lyophilized powder)

1x0.5mg α-cardiac actin (lyophilized powder)

1x50µg Vinculin tail

5x50ml MonoMix, (lyophilized G-actin ATP-buffer)

1x1.0ml PolyMix 10x stock (lyophilized F-actin ATP-buffer, pH 7.4)

1x0.5ml 1M MgCl2

1x Toolkit Handbook Fluorescence Microscopy

10x Light protective tubes & tags

Actin Buffer

2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3%

disaccharides, when reconstituted with 1.0 ml ultrapure water to obtain a 1mg/ml

solution.

Purification notes

Purified from rabbit skeletal muscle, GPC.

Protein concent.

Determined by the Biuret method

Storage instructions

We guarantee stable performance of the kit components for 6 months when stored at

-70°C upon arrival. Solubilized G-actin is kept on ice and should be used within 1

week. Avoid refreezing.

Shipping conditions

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or

Therapeutical Applications.

CAS no.

51005-14-2 (Actin)

Fluorescence Microscopy of Atto550-Actin filament

bundles, induced by vinculin tail (Vt). This assay

shows the actin bundling properties of the ligand.(Vt).

SDS-PAGE of a low speed cosedimentation.

The "Actin-Toolkit F-Actin Binding" demonstrate

the actin bundling activity of the vinculin tail(Vt) at

low speed centrifugation. Upon addition of Vt to

F-Actin, the Vt-Actin complexes are pelleted, while

F-Actin and Vt alone remain in the supernatant.

Protein

Atto488-Actin (α-Actin)

Origin

Skeletal muscle alpha actin, rabbit

Molecular mass

42kDa

Toolkit description

The Actin-Toolkit Fluorescence Microscopy was developed to analyze and

demonstrate the effect of ligands on actin polymerization, or ligand-induced

formation of actin bundles and networks. In the assays, Atto488-Actin

(λem 488nm, λem 523nm) is mixed with the ligand to observe the effects on

either G- or F-actin. In contrast to staining with phallodin conjugates, which can

interfere with ligand binding sites on the actin molecule, no such observations

were made with Atto labeled actin. Atto-Actins are validated to possess the

polymerization properties of native actin.

The Toolkit further contains unlabeled actin (0.5mg α-skeletal muscle actin).

A mixture of both excludes possible interference of the fluorescent actin conjugates

on ligand binding sites. Vinculin tail - a V8 protease cleavage

product of vinculin - is an efficient actin bundling vinculin domain, is supplied

with the Toolkitas a bundling reference.

several standardized experiments are described step-by-step in the handbook

supplied with the kit. In combination with the actin buffers contained in the kit,

error-free handling is guaranteed. The kit contains 4x100µg of α-skeletal of

α-skeletal muscle Atto488-Actin conjugates and buffers to keep the actin functional.

Purity

 >99% by scanning densitometry

Form

Lyophilized, ready-to-use

Kit content

4x100µg Atto488-Actin (lyophilized powder)

1x0.5mg α-skeletal muscle actin (lyophilized powder)

1x50µg Vinculin tail

5x50ml MonoMix, (lyophilized G-actin ATP-buffer)

1x1.0ml PolyMix 10x stock (lyophilized F-actin ATP-buffer, pH 7.4)

1x0.5ml 1M MgCl2

1x Toolkit Handbook Fluorescence Microscopy

10x Light protective tubes & tags

Actin Buffer

2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3%

disaccharides, when reconstituted with 1.0 ml ultrapure water to obtain a 1mg/ml

solution.

Purification notes

Purified from rabbit skeletal muscle, GPC.

Protein concent.

Determined by the Biuret method

Storage instructions

We guarantee stable performance of the kit components for 6 months when stored at

-70°C upon arrival. Solubilized G-actin is kept on ice and should be used within 1

week. Avoid refreezing.

Shipping conditions

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or

Therapeutical Applications.

CAS no.

51005-14-2 (Actin)