Products

Protein

Actin (α-Actin)

Origin

skeletal muscle alpha actin, bovine

Molecular mass

42kDa

Toolkit description

Non-Polymerizing Actin is a-skeletal muscle actin (42kD,  375 aa), enzymatically

cleaved between Gly42/Val43. As a result of this cleavage an essential

monmer-monomer interaction site is inactivated, rendering the actin

unpolymerizable in the presence of Ca++ and e.g. 100mM KCl, conditions where

normal actin polymerizes.

Thus, NP-actin is a valuable tool for G-actin-ligand studies in the presence of

Ca++ and KCl. On the other hand NP-actin remains polymerizable in the presence

Mg++, with a critical concentration of 3µM (barbedend).

Actin compatible buffers preserve the structural integrity of non-polymerizing actin,

while its cleavage products (approx. 36kD and 6kD) are only obtained under

denaturing conditionsm e.g. SDS-PAGE.

Purity

 >99% by scanning densitometry

Form

Lyophilized, ready-to-use

Quantity

1.0 mg

Buffer

2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3%

disaccharides, when reconstituted with 1.0 ml ultrapure water to obtain a 1mg/ml

solution.

Purification notes

Purified from rabbit skeletal muscle, GPC.

Protein conc.

Determined by the Biuret method.

Storage instructions

NP-Actin stored at -70°C upon arrival will be stable in performance for at least

6 months from the date of purchase. Solubilized G-actin is kept on ice and

should be used within 1 week. Avoid refreezing.

Shipping conditions

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or

Therapeutical Applications.

CAS no.

51005-14-2