화학합성의 각종 TRP Channel Blocker Peptide Toxin (1)
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화학합성의 각종 TRP Channel Blocker Peptide Toxin (1)
TRP channel 연구에 유용한 유독 생물 유래의 합성 peptide toxin 입니다. 화학합성 peptide이기 때문에
endotoxin 오염의 걱정이 없습니다.
GsMTx4
GsMTx4 has been isolated from the venom of the spider Grammostola rosea. This cationic hydrophobic polypeptide blocks
mechanosensitive ion channels such as TRPC1 or TRPC6, without having any effect on whole-cell voltage-sensitive
currents. This toxin acts by perturbing the interface between the channel and the lipid bilayer without necessarily being
in physical contact with the channel. GsMTx4 also demonstrated to active TRPA1 channel at 1µM concentration.
Details
|
AA sequence: |
|
H-Gly-Cys2-Leu-Glu-Phe-Trp-Trp-Lys-Cys9-Asn-Pro-Asn-Asp-Asp-Lys-Cys16-Cys17-Arg-Pro-Lys- Leu-Lys-Cys23-Ser-Lys-Leu-Phe-Lys-Leu-Cys30-Asn-Phe-Ser-Phe-NH2 |
|
|
|
(Disulfide bonds between Cys2-Cys17, Cys9-Cys23 & Cys16-Cys30 ) |
|
Length (aa): |
|
34 |
|
Formula: |
|
C185H273N49O45S6 |
|
Molecular Weight: |
|
4095.2 Da |
|
Appearance: |
|
White lyophilized solid |
|
Solubility: |
|
Water and saline buffer |
|
CAS number: |
|
Not available |
|
Source: |
|
Synthetic |
|
Purity rate: |
|
> 97% |
Citration
1. Anderson M, et al. (2013) Opposing effects of podocin on the gating of podocyte TRPC6 channels evoked by
membrane stretch or diacylglycerol. Am J Physiol Cell Physiol. PubMed link
References
1. Bae, C., et al. (2011) The Mechanosensitive Ion Channel Piezo1 Is Inhibited by the Peptide GsMTx4. Biochemistry.
2. Kerstin Hill, Michael Schaefer (2007) TRPA1 Is Differentially Modulated by the Amphipathic Molecules Trinitrophenol
and Chlorpromazine. JBC. PubMed link
3. Ruta, V., and MacKinnon, R. (2004) Localization of the voltage-sensor toxin receptor on KvAP, Biochemistry.
3. Ruta, V., and MacKinnon, R. (2004) Localization of the voltage-sensor toxin receptor on KvAP, Biochemistry.
4. Ostrow, K. L., et al. (2003) cDNA sequence and in vitro folding of GsMTx4, a specific peptide inhibitor of
mechanosensitive channels. Toxicon. PubMed link
5. Oswald, R. E., et al. (2002) Solution structure of peptide toxins that block mechanosensitive ion channels.
J Biol Chem. PubMed link
6. Suchyna, T. M., et al. (2000) Identification of a peptide toxin from Grammostola spatulata spider venom that blocks
cation-selective stretch-activated channels. J Gen Physiol. PubMed link
Vanillotoxin-3 (VaTx3)
Vanillotoxin-3 (VaTx3) has been isolated from the venom of Psalmopoeus cambridgei, a West Indies tarantula. It is a 34
amino acids peptide with three disulfide bridges with an ICK motif. It selectively activates mammalian TRPV1 (capsaicin
receptor), a non-selective cation channel expressed by sensory neurons of the pain pathway with an EC50 value of
0.45 ± 0.04 µM. It is more potent than VaTx1 (20-fold) and VaTx2 (2-fold), two other analogues of VaTx3 with closely
related sequences and isolated from the same venom. VaTx3 probably interacts with distinct regions of the channel
than capsaicin. VaTx3 does not affect TRPV2, TRPV3, TRPV4, TRPA1, TRPM8, Kv1.2 (KCNA2), Kv2.1 (KCNB1) or Kv4.2 (KCND2). In mice, VaTx3 elicits pain-related behaviors, such as licking and flinching of the affected limb. Edema is
evidenced in the paw of toxin-injected mice.
Details
|
AA sequence: |
|
Glu-Cys2-Arg-Trp-Tyr-Leu-Gly-Gly-Cys9-Lys-Glu-Asp-Ser-Glu-Cys15-Cys16-Glu-His-Leu-Gln-
Cys21-His-Ser-Tyr-Trp-Glu-Trp-Cys28-Leu-Trp-Asp-Gly-Ser-Phe-NH2 |
|
|
|
(Disulfide bonds between Cys2-Cys16, Cys9-Cys21 & Cys15-Cys28 ) |
|
Length (aa): |
|
34 |
|
Formula: |
|
C184H241N47O55S6 |
|
Molecular Weight: |
|
4179.6 Da |
|
Appearance: |
|
White lyophilized solid |
|
Solubility: |
|
Water and saline buffer |
|
CAS number: |
|
Not available |
|
Source: |
|
Synthetic |
|
Purity rate: |
|
> 98% |
References
1. Siemens J., et al. (2006) Spider toxins activate the capsaicin receptor to produce inflammatory pain. Nature.
2. Brett A., et al. (2008) Painful toxins acting at TRPV1. Toxicon. PubMed link
Ordering informations
|
Catalog No. |
Product Name |
Size |
|
08GSM001 |
GsMTx4 |
0.05mg, 0.1mg & 0.5mg |
|
10VAN003 |
VaTx3 |
0.05mg, 0.1mg & 0.5mg |
* 다른 사이즈나 bulk 공급도 가능하오니 별도로 문의하여 주십시오.
▣ 관련 페이지 ; Smartox Biotechnology
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GaTx1 / GaTx2 / Chlorotoxin
• 화학 합성의 각종 Calcium Channel Blocker Peptide Toxin
ω-agatoxin IVA / ω-CONOTOXIN GVIA/Omega conotoxin GVIA / ω-Conotoxin MVIIC/Omega conotoxin MVIIC / SNX482/
Maurocalcine/Huwentoxin I/ω CONOTOXIN SO-3/Omega contoxin SO-3/ω-Conotoxin MVIIA/Omega conotoxin MVIIA
• 화학 합성의 각종 Potassium Channel Blocker Peptide Toxin
Iberiotoxin (IbTx) / Charybdotoxin / Leiurotoxin 1 / Tamapin / Guangxitoxin 1E / ShK (Stichodactyla toxin)/
Margatoxin / HsTx1 / Apamin / TERTIAPIN-Q / Maurotoxin / Kaliotoxin-1
• 화학 합성의 각종 Sodium Channel Blocker Peptide Toxin
Protoxin I (ProTx-1) / GsAF-I / GsAF-II / Jingzhaotoxin III / Biotinyl-Protoxin II (ProTx II) / Hainantoxin-IV /
Protoxin II (ProTx II) / Huwentoxin IV / Huwentoxin I / μ Conotoxin PIIIA / mu conotoxin PIIIA
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