| Human Chimeric monoclonal to SARS-CoV-2 (2019-nCov) Anti-SARS-CoV-2 (2019-nCov) Nucleocapsid Chimeric Human Monoclonal Antibody [RMH03]  | Product Details | | Product Name | Anti-SARS-CoV-2 (2019-nCov) Nucleocapsid Chimeric Human Monoclonal Antibody [RMH03] | | Product Description | This antibody is a Human-Rabbit chimeric antibody, made by fusion of the antigen binding region (variable domains of the heavy and light chains, VH and VL) of a rabbit anti-NP(2019-nCov) monoclonal antibody, with the constant domain of Human IgG1. This antibody is recognized only by an anti-Human IgG secondary antibody. | | Clone Name | RMH03 | | Produt Data Sheet | Anti-SARS-CoV-2 (2019-nCov) Nucleocapsid Chimeric Human Monoclonal Antibody [RMH03] (PDF) | | Specificity | RMH03 reacts 2019-nCov Nucleocapsid Protein. | | Immunogen | A recombinant 2019-nCov Nucleocapsid Protein expressed in E.coli. | | Application | Sandwich ELISA assay as a detection antibody, | | pair with rabbit monoclonal anti-2019-nCov NP clone RM420 | | Species Reactivity | Validated in COVID-19 patient samples. | | Conjugate | None | | Sensitivity | The binding activity of N protein with anti-N protein antibody was verified with the sensitivity of 0.1-0.3 ng/mL. | | Specificity | No cross-reactivity to common SARS coronaviruses. |
| Properties | | | Form | Liquid | | Storage Instructions | Store at -20°C | | Stability | Stable for 1 Year at -20.0°C from date of receipt | | Storage Buffer | PBS only | | Volume/Item Size | 100 ug /concentration : 1 mg/ml | | Purity | Protein A affinity purified from an animal origin–free culture supernatant | | Clone Type | Monoclonal | | Isotype | Human IgG1 |
 Binding curve of anti-NP (2019-nCov) RMH03 and recombinant 2019-nCov Nucleocapsid Protein. ELISA plate was coated with 50 ng recombinant 2019-nCov Nucleocapsid Protein at concentration of 1ug/mL. A 2-fold serial dilution from 125 ng/mL was performed using RMH03. For detection, AP-conjugated goat anti-human IgG secondary antibody was used. |