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TMR-Actin
Polymerization impossible
High G-actin concentrations works...
TMR-Actin is native G-actin covalently modified with tetramethylrhodamine 5-maleimide at Cys374. TMR-
Actin from Hypermol is tested as supplied to be monomeric up to 20mg/ml.
Ready-to-use.
TMR-Actin (alpha actin, rabbit skeletal muscle)
Description
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Protein
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TMR-Actin
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Origin
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Skeletal muscle alpha actin, rabbit
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Molecular mass
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42kDa
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Protein description
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G-actin from rabbit skeletal muscle is a single chain polypeptide with a molecular
weight of 42kD consisting of 375 amino acids. TMR-Aactin is chemically modified
G-actin, carrying a tetramethlyrhodamine group covalently bound to Cys374 at the
C-term of the molecule.
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Conjugation ratio
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TMR-Actin is supplied with a DOL=0.9-1 (DOL -Degree of labelling).
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Properties
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Form
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Lyophilized, ready-to-use
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Quantity
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0.5 mg
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Buffer
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2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3%
disaccharides, when reconstituted with 1.0 ml ultrapure water to obtain a 1mg/ml
solution.
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Purity
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99% by scanning densitometry.
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Purification notes
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Purified from rabbit skeletal muscle.
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Protein conc.
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Determined by the Biuret method.
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Storage instructions
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TMR-actin is stored at -70°C upon arrival and will be stable in performance for
at least 6 months from the date of purchase. Solubilized pyrene actin is kept on
ice and should be used within 1 week. Avoid refreezing.
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Shipping conditions
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At ambient temperature. Upon delivery store at -70°C.
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