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Isolation of microsomal membranes from plant tissues is a common laboratory procedure. Microsomal

fraction of plant cell lysate is the focus of interest in many plant research projects. Microsomal fraction is

believed to be enriched for plasma membranes, endoplasmic reticulum, Golgi apparatus, vacuolar

membranes and other components of membrane system. Traditional method for microsomal fraction

isolation involves so called differential pelleting protocol where a series of centrifugation stepsare required to

obtain various membrane fractions. Traditional protocol for microsomal fraction isolation requires large

amount of starting material and employs tedious ultracentrifugation steps. MM-018 offers a simple, rapid and

user friendly approach for microsomal membrane extraction using small amount of starting material (200 mg)

. Water soluble cytosolic proteins are removed during the procedures and water insoluble microsomal

fraction, especially plasma membrane fraction, is extracted with optimized buffersin a table-top

microcentrfuge. The procedure is simple, rapid and no special instrument required. Native microsomal

proteins can be isolated from plant tissue in about one hour without ultracentrifugation. The protein yield is in

the range of 100-200 µg/sample.