Products

Mouse Embryonic Tissue cDNA-E10, E12 & E14

Zaygen offer full-length  first strand cDNA of many embryonic organs (tissues) harvested from embryos at

different stages of development. The ready-to-use cDNA is synthesized from high quality RNA extracted from

wide variety of embryonic tissues at developmental stages. Embryonic tissues are collected from frozen

embryo sections mounted on slides by scraping each tissue by fine forceps or needles under dissecting

microscope. Total RNA is then extracted using a modified guanidine thiocyanate-phenol/chloroform method,

treated with RNase-free DNase to remove residual DNA and subjected for cDNA synthesis. The cDNA is

primed with oligo dT primer using reverse transcriptase enable full length cDNA synthesis and is ideal for

gene discovery and expression analysis applications. As quality control test, each cDNA sample is tested

by PCR amplification of beta-actin. Each product is shipped in 1.5 ml tube and is enough for 30 PCR

reactions.

Custom cDNA Isolation Services: Zyagen also offers custom service to prepare ready to use cDNA of any

mouse embryonic tissue at any stage of development.  Contact customer services/technical support for

more information.