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Actin Binding Protein, Alpha-Actinin (Smooth muscle turkey)
Calcium insensitive bundling and cross-linking.
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Alpha-Actinin is a homodimeric organizer of
the cytoskeleton, beloning to the spectrin
superfamily.
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Alpha-Actinin (Actin Binding Protein) - 100 µg
Description
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Protein
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α-Actinin
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Origin
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Smooth muscle, turkey
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Molecular mass
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~200kDa
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Protein description
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Native α-Actinin from smooth muscle has a molecular mass of 200kDa and forms
homodimers (2 x 100kDa).
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Actin interaction
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Actin cross linking protein. Kd actin-binding = 1-5 µM.
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Properties
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Form
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Lyophilized, ready-to-use
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Quantity
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100 µg
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Buffer
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50mM NaCl, 20mM Tris pH 7.4, 0.5mM DTT and 3% disaccharides, when
reconstituted with 1.0µl ultrapure water to obtain a 1mg/ml solution.
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Purity
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>95% by scanning densitometry from coomassie G-250 stained SDS-Gels.
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Purification notes
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Purified from turkey gizzard muscle.
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Protein conc.
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Determined UV-VIS (A280=0.1201)
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Storage instructions
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Upon reconstitution the product is stored on ice. This product can also be stored as
a glycerol stock at -20°C. Avoid repeated freeze / thaw cycles.
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Shipping conditions
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At ambient temperature. Upon delivery store at -70°C.
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Remarks
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For Use in Research only. Not for Use in Human or Veterinary Diagnostical or
Therapeutical Applications.
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Alpha-Actinin (Actin Binding Protein) - 1.0 mg
Description
|
Protein
|
|
α-Actinin
|
|
Origin
|
|
Smooth muscle, turkey
|
|
Molecular mass
|
|
~200kDa
|
|
Protein description
|
|
Native α-Actinin from smooth muscle has a molecular mass of 200kDa and forms
homodimers (2x100kDa).
|
|
Actin interaction
|
|
Actin cross linking protein. Kd actin-binding = 1-5 µM.
|
Properties
|
Form
|
|
Lyophilized, ready-to-use
|
|
Quantity
|
|
1.0 mg
|
|
Buffer
|
|
25mM NaCl, 20mM Tris pH 7.4, 0.5mM DTT and 3% disaccharides, when
reconstituted with 1.0µl ultrapure water to obtain a 1mg/ml solution.
|
|
Purity
|
|
>95% by scanning densitometry from coomassie G-250 stained SDS-Gels.
|
|
Purification notes
|
|
Purified from turkey gizzard muscle.
|
|
Protein conc.
|
|
Determined UV-VIS (A280=0.1201)
|
|
Storage instructions
|
|
Upon reconstitution the product is stored on ice. This product can also be stored as
a glycerol stock at -20°C. Avoid repeated freeze / thaw cycles.
|
|
Shipping conditions
|
|
At ambient temperature. Upon delivery store at -70°C.
|
|
Remarks
|
|
For Use in Research only. Not for Use in Human or Veterinary Diagnostical or
Therapeutical Applications.
|
|