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Actin Binding Protein, Arp 2/3
The complex machinery.
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Arp2/3 is the natively prepared seven subunit
complex. It is supplied as a highly purified and
soluble protein complex and is ready-to-use.
Arp2/3 is shipped in a stable form as lyophilized
protein, fully functional after reconstitution.
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Arp 2/3 (Actin Binding Protein) - 50 µg
Description
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Protein
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Arp 2/3 complex
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Origin
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brain, porcine
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Molecular mass
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224kDa
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Protein description
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The seven-subunit protein complex comprises Arp2 and Arp3 and five smaller
(Arc) proteins. Both Arp2 and Arp3 sequences possess nucleotide-binding and
divalent cation-binding sites. Arp 2/3 complex consists of five associated proteins,
namely p16, p20, p21, p34, and p40 (Synonyms: ARPC-5, -4, -3, -2, and -1.)
Arp 2/3 is a constitutively inactive complex. Nucleation is efficiently activated
In vitro by binding of the VCA-domains of members of the Wiskott-Aaldrich
syndrome family protein, like WAVE (WASP family verprolin homologous).
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Actin interaction
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Pointed end capping protein. Branching of actin filaments. Strong Nucleation upon
activation by VCA-domain (cat. #.: 8416-01)
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Properties
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Form
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Lyophilized, ready-to-use
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Quantity
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50 µg
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Buffer
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150mM KCl, 20mM Trizma pH 7.5, 1mM DTT, 0.2mM ATP, 0.5mM EDTA,
1mM MgCl2 and 5% disaccharides, when reconstituted with 100µl ultrapure
water to obtain a 0.5mg/ml solution.
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Purity
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>90% by scanning densitometry from Coomassie G-250 stained SDS-Gels.
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Purification notes
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Purified from porcine brain.
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Protein conc.
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Determined at OD280 (0.1%=1.033).
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Storage instructions
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Arp2/3 complex is stored at -70°C. upon arrival will be stable in performance for
at least 6 months from the date of purchase. The solubilized Arp2/3 complex is kept
on ice and should be stored at -20°C in glycerol or CryoProtect (Cat.#: 6011-01).
Avoid repeated freeze / thaw cycles.
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Shipping conditions
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At ambient temperature. Upon delivery store at -70°C.
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Remarks
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For Use in Research only. Not for Use in Human or Veterinary Diagnostical or
Therapeutical Applications.
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Arp 2/3 (Actin Binding Protein) - 1.0 mg
Description
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Protein
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|
Arp 2/3 complex
|
|
Origin
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brain, porcine
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Molecular mass
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224kDa
|
|
Protein description
|
|
The seven-subunit protein complex comprises Arp2 and Arp3 and five smaller
(Arc) proteins. Both Arp2 and Arp3 sequences possess nucleotide-binding and
divalent cation-binding sites. Arp 2/3 complex consists of five associated proteins,
namely p16, p20, p21, p34, and p40 (Synonyms: ARPC-5, -4, -3, -2, and -1.)
Arp 2/3 is a constitutively inactive complex. Nucleation is efficiently activated
In vitro by binding of the VCA-domains of members of the Wiskott-Aaldrich
syndrome family protein, like WAVE (WASP family verprolin homologous).
|
|
Actin interaction
|
|
Pointed end capping protein. Branching of actin filaments. Strong Nucleation upon
activation by VCA-domain (cat. #.: 8416-01)
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Properties
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Form
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Lyophilized, ready-to-use
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Quantity
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1.0mg
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Buffer
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150mM KCl, 20mM Trizma pH 7.5, 0.2mM ATP, 0.5mM EDTA, 1mM MgCl2
and 5% disaccharides, when reconstituted with 1.0µl ultrapure water to obtain
a 1mg/ml solution.
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Purity
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>90% by scanning densitometry from Coomassie G-250 stained SDS-Gels.
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Purification notes
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Purified from porcine brain.
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Protein conc.
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Determined at OD280 (0.1%=1.033).
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|
Storage instructions
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|
Arp2/3 complex is stored at -70°C. upon arrival will be stable in performance for
at least 6 months from the date of purchase. The solubilized Arp2/3 complex is kept
on ice and should be stored at -20°C in glycerol or CryoProtect (Cat.#: 6011-01).
Avoid repeated freeze / thaw cycles.
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|
Shipping conditions
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|
At ambient temperature. Upon delivery store at -70°C.
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|
Remarks
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For Use in Research only. Not for Use in Human or Veterinary Diagnostical or
Therapeutical Applications.
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