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Actin Binding Protein, Filamin
The secret of Flexibility
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... from turkey gizzard is a homodimer, consisting of
24 repeats of ~96 amino acids and an N-terminal
actin binding sequence.
Filamin crosslinks actin filaments into orthogonal
networks.The C-terminal end of each Filamin
monomer is characterized by a hinge region,
responsible for the flexibility of actin networks.
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Actin networks
Filamin : Atto488-Actin ( 1:12 M/M)
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Actin Binding Protein
Description
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Protein
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Filamin (Filamin A)
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Origin
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smooth muscle, turkey
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Molecular mass
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546kDa
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Protein description
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Filamin from turkey gizzard is a homodimer (2x273kDa) with a molecular mass of
546kDa, consisting of 24 repeats of ~96 amino acids and an N-terminal actin binding
sequence. Filamin crosslinks actin filaments into orthogonal networks.
The C-terminal end of each Filamin monomer is characterized by a hinge region,
responsible for the flexibility of actin networks.
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Actin interaction
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Actin cross linking protein. Kd actin-binding = 0.5µM.
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Properties
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Form
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Lyophilized, ready-to-use
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Quantity
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100 µg
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Buffer
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10mM NaCl, 20mM Tris pH 7.4, 0.5mM DTT and 2.4% disaccharides,
when reconstituted with 1.0ml ultrapure water to obtain a 1.0 mg/ml solution.
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Purity
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>95% by scanning densitometry from Coomassie G-250 stained SDS-Gels.
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Purification notes
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Purified from turkey gizzard muscle.
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Protein conc.
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Determined by OD280 (0.1%=0.64).
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Storage instructions
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upon reconstitution the product is stored on ice. This product can also be stored as
a glycerol stock at -20°C. Avoid repeated freeze / thaw cycles.
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Shipping conditions
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At ambient temperature. Upon delivery store at -70°C.
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Remarks
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For Use in Research only. Not for Use in Human or Veterinary Diagnostical or
Therapeutical applications.
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Actin Binding Protein
Description
|
Protein
|
|
Filamin (Filamin A)
|
|
Origin
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|
Smooth muscle, turkey
|
|
Molecular mass
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|
546kDa
|
|
Protein description
|
|
Filamin from turkey gizzard is a homodimer (2x273kDa) with a molecular mass of
546kDa, consisting of 24 repeats of ~96 amino acids and an N-terminal actin binding
sequence. Filamin crosslinks actin filaments into orthogonal networks.
The C-terminal end of each Filamin monomer is characterized by a hinge region,
responsible for the flexibility of actin networks.
|
|
Actin interaction
|
|
Actin cross linking protein. Kd actin-binding = 0.5µM.
|
Properties
|
Form
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|
Lyophilized, ready-to-use
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|
Quantity
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1.0mg
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Buffer
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|
10mM NaCl, 20mM Tris pH 7.4, 0.5mM DTT and 2.4% disaccharides,
when reconstituted with 1.0ml ultrapure water to obtain a 1.0 mg/ml solution.
|
|
Purity
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|
>95% by scanning densitometry from Coomassie G-250 stained SDS-Gels.
|
|
Purification notes
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|
Purified from turkey gizzard muscle.
.
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Protein conc.
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|
Determined by OD280 (0.1%=0.64).
|
|
Storage instructions
|
|
upon reconstitution the product is stored on ice. This product can also be stored as
a glycerol stock at -20°C. Avoid repeated freeze / thaw cycles.
|
|
Shipping conditions
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|
At ambient temperature. Upon delivery store at -70°C.
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|
Remarks
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For Use in Research only. Not for Use in Human or Veterinary Diagnostical or
Therapeutical applications.
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