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Actin Binding Protein, Tropomyosin
Strengthening & regulating coiled-colis.
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Tropomyosin from skeletal muscle consists of rod-shpaed
coiled-coil dimers (hetero-or homo dimers in head-to-tail
association). The Tropomyosin dimers bind laterally to the
actin filament - along the α-helical groove of the filaments.
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Tropomyosin
Description
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Protein
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Tropomyosin
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Origin
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Skeletal muscle, rabbit
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Molecular mass
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~70kDa
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Molecular structure
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Heterodimeric protein (α,β), consisting of alpha helical coiled coils.
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Actin interaction
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Laterally binds to F-actin at a molar ratio of 1:7 (tropomyosin:g-actin).
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Properties
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Form
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Lyophilized, ready-to-use
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Quantity
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2 x 100 µg & 1 x 500 µg
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Buffer
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0.05M KCl, 20mM imidazole pH 7.4, 1mM EDTA, 1mM DTT and 2.5% disaccharides,
when reconstituted with ultrapure water to obtain a 1mg/ml solution.
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Purity
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>98% by scanning densitometry.
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Purification notes
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Purified from rabbit skeletal muscle.
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Protein conc.
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Determined by the Biuret method.
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Storage instructions
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Upon reconstitution the product is stored on ice. This product can also be stored
as a glycerol stock at -20°C. Avoid repeated freeze / thaw cycles.
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Shipping conditions
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At ambient temperature. Upon delivery store at -70°C.
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Remarks
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For Use in Research only. Not for Use in Human or Veterinary Diagnostical or
Therapeutical applications.
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