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Actin Binding Protein, Tropomyosin-Troponin
Regulators of Contraction.
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Tropomyosin/Troponin is a complex consisting of
tropomyosin, troponin C, troponin I, and troponin T, that
regulates Myosin II interaction with actin in striated
muscles (sliding filament model)..
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Tropomysin / Troponin
Description
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Protein
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Tropomyosin-Troponin (Synonym: native Tropomyosin)
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Origin
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Skeletal muscle, rabbit
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Molecular mass
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~148kDa
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Molecular structure
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Tropomyosin (2x35kDa), Troponin T (37kDa), Troponin I (23kDa), Troponin C (18kDa)
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Actin interaction
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Ca++-sensitive, regulatory laterally F-actin binding complex. Molar ratio = 1:7
(tropomyosin/troponin:g-actin).
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Properties
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Form
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Lyophilized, ready-to-use
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Quantity
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2 x 100 µg
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Buffer
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0.1M KCl, 20mM imidazole pH 7.4, 1mM EDTA, 2mM DTT and 2% disaccharides,
when reconstituted with 100µl ultrapure water to obtain a 1mg/ml solution.
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Purity
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>98% by scanning densitometry.
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Purification notes
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Purified from rabbit skeletal muscle.
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Protein conc.
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Determined by the Biuret method.
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Storage instructions
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Tropomyosin/Troponin is stored at -70°C upon arrival will be stable in performance
for at least 6 months from the date of purchase.
The Solubilized complex is kept on ice and should be used within 1 week.
This product can also be stored as a glycerol stock at -20°C. Avoid repeated
freeze / thaw cycles.
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Shipping conditions
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At ambient temperature. Upon delivery store at -70°C.
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Remarks
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For Use in Research only. Not for Use in Human or Veterinary Diagnostical or
Therapeutical applications.
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