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New ! Anti-Mouse IgM Atto490LS (H+L)

New! Anti-Mouse IgM Atto490LS (H+L)

Highly specific secondary antibodies - strong & long lasting fluorescence!

매우 안정한 dyes에 conjugate한 항체로, 매우 높은 affinity를 갖고 있습니다.

The quality of secondary antibodies is essential in producing superior results in immunofluorescence.

Hypermol offers secondary antibodies conjugated to the novel, high quality Atto dyes for detecting primary

antibodies.

Atto-labeled antibodies belong to the new generation of fluorescent antibodies characterized by their

exceptional fluorescence intensity and superior photostability. For this reason they are suited for all kinds of

immunofluorescence microscopy and especially for high and super-resolution techniques.

Compared to established fluorescent antibodies, Atto antibodies show a remarkably prolonged fluorescence,

as a result of the outstanding features of the dyes, as demonstrated by two examples:

1. Atto 647N fluoresces in aqueous solution twice as strong as Cy5TM.

2. under identical conditions Atto 647N lasts up to 100 times longer than dyes like Cy5TMand Alexa 647TM.

Atto-conjugated IgGs from Hypermol are affinity purified and quality-control tested by UV-VIS spectroscopy

and immunocytochemistry. They come as convenient kits with a well characterized, epitope mapped actin

antibody for reference, double staining or ligand analysis, with a detailed description in the product

documentation of each kit.

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Anti-Mouse IgM Atto490LS (H+L), Antibody produced in goat

Fluorescent Antobody kit

Description

Product name		Anti-Mouse IgM Atto490LS
Target species		Mouse
Description		Atto-labeled secondary antibodies belong to the new generation of fluorescent antibodies, characterized by their exceptional fluorescence intensity and superior photostability. Anti-Mouse IgM Atto490LS has a very large stokes shift (165nm). It is thus is specifically suited for suited for all multiplexing approaches, especially in combination with Atto488 and Atto514 conjugated antibodies. For convenient reference, Fluorescent Antibody Kits contain gratis a primary epitope mapped actin antibody of high performance also on western blots.
Specifications		Goat anti-rabbit IgG Atto490 (H+L), Immunofluorescence: 1:300-1:1100, Degree of labeling (DOL) : 2-9, Unconjugated dye ≤5% of total fluorescence. For further information please see DataSheet.
Optical properties		λex 496nm, λem 661nm

Properties

Form		Liquid, ready-to-use
Concentration		2mg/ml
Content		1mg Anti-Mouse IgM (H+L) Atto490LS 50µg (1mg/ml) monoclonal anti-actin 1ml Glycerol buffer Product documentation Certificate of Analysis
Buffer		50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, 10 mM sodium azide, pH 7.4.
Clonality		Polyclonal
Isotype		IgM
Purification notes		Column affinity purified
Storage condition		Store as glycerol stock at -20°C. Avoid repeated freeze / thaw cycles.
Shipping condition		Shipped at ambient temperature
Remarks		For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications.

Secondary Antibodies series

Anti-Mouse IgG Atto

Anti-Mouse IgM Atto

Anti-Rabbit IgG Atto

Ordering informations

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