Products

Topoisomerase DNA cleavage assays

For topoisomerase inhibition mechanism and drug toxicity tests

Bacterial gyrase, topoisomerase IV (parC-parE ) and mammalian topoisomerase II are type II

topoisomerases. These topoisomerases change DNA topology by cleavage and religation of both DNA

strands. When an inhibitor such as fluoroquinolone drugs blocks the religation step of the topoisomerase

reaction, the cleaved or linearized DNA forms covalent complexes with the enzyme (cleavage complex).

Formation of DNA cleavage complexes causes DNA damage in the cells (topoisomerase poison). Testing

formation of DNA cleavage complexes is useful for understanding the topoisomerase inhibition mechanism of

novel topoisomerase inhibitors. It is also useful for toxicity evaluation regarding to human topoisomerase II.

Topoisomerase DNA cleavage assay kit (enzyme not included)   

ProFoldin’s topoisomerase DNA cleavage assay kit is for testing DNA cleavage complex formation of type II

topoisomerases (gryase, parC-parE, mammalian topo II) by topoisomerase-poisoning compounds. The

assay is based on the principle that the linearized or cleaved DNA has much greater permeability through a

solution matrix and a filter than the closed circular DNA.  The assay kit (TDC96K) is in 96-well plate format

and suitable for testing one or many compounds at a time. The kit includes the reaction buffer, DNA

substrate, protease digestion solutions, solution matrix, rinse buffer, DNA detection dye and a filter plate.

Topoisomerase is not included in the kit but can be purchased separately. A plate filtration device and a

vacuum line are needed for the plate filtration process.

E. coli Gyrase DNA Cleavage Assay Kit Plus (enzyme included)  

ProFoldin’s topoisomerase DNA cleavage assay kit is for testing DNA cleavage complex formation of type II

topoisomerases (gryase, parC-parE, mammalian topo II) by topoisomerase-poisoning compounds. The

assay is based on the principle that the linearized or cleaved DNA has much greater permeability through a

solution matrix and a filter than the closed circular DNA.  The assay kit (T2C96KE) is in 96-well plate format

and suitable for testing one or many compounds at a time. The kit includes the reaction buffer, DNA

substrate, E. coli gyrase, protease digestion solutions, solution matrix, rinse buffer, DNA detection dye and a

filter plate. A plate filtration device and a vacuum line are needed for the plate filtration process.

E. coli Topo IV DNA Cleavage Assay Kit Plus

ProFoldin’s topoisomerase DNA cleavage assay kit is for testing DNA cleavage complex formation of type II

topoisomerases (gryase, parC-parE, mammalian topo II) by topoisomerase-poisoning compounds. The

assay is based on the principle that the linearized or cleaved DNA has much greater permeability through a

solution matrix and a filter than the closed circular DNA.  The assay kit (T4C96KE) is in 96-well plate format

and suitable for testing one or many compounds at a time. The kit includes the reaction buffer, DNA

substrate, E. coli topoisomerase IV, protease digestion solutions, solution matrix, rinse buffer, DNA detection

dye and a filter plate. A plate filtration device and a vacuum line are needed for the plate filtration process.  

S. aureus Topo IV DNA Cleavage Assay Kit Plus (enzyme included)

ProFoldin’s topoisomerase DNA cleavage assay kit is for testing DNA cleavage complex formation of type II

topoisomerases (gryase, parC-parE, mammalian topo II) by topoisomerase-poisoning compounds. The

assay is based on the principle that the linearized or cleaved DNA has much greater permeability through a

solution matrix and a filter than the closed circular DNA.  The assay kit (T4C96KS) is in 96-well plate format

and suitable for testing one or many compounds at a time. The kit includes the reaction buffer, DNA

substrate, S. aureus topoisomerase IV, protease digestion solutions, solution matrix, rinse buffer, DNA

detection dye and a filter plate. A plate filtration device and a vacuum line are needed for the plate filtration

process.