Products

Detergent Removal Systems

Choose from  a variety of detergent removal systems.

The Detergent-OUT™ is a simple and high performance method for removing a wide variety of detergents,

such as Triton, NP-40, Tween-20, CTAB, CHAPS, Lubrol and deoxycholate from protein solutions.

Detergent-OUT™ removes detergents without significant loss of proteins, dilution of the protein solution, or

change to the buffer composition of the protein solution.

Our Detergent-OUT™ SDS-300 kit is a simple method for removing free detergents from protein solutions with

higher performance than equilibrium dialysis, electro-dialysis or other ion exchange methods. This method

removes free detergents without substantial loss or dilutions of the protein solution. The Detergent-OUT™

method is capable of achieving molar ratio of protein: SDS to 1:1.

The OrgoSol Detergent-OUT™ kit has been specifically developed for removing all types of detergent from

protein solution. OrgoSolDetergentOUT™ can be used for removing ionic, non-ionic and cationic detergents

and also suitable for removing detergents from hydrophobic proteins.

Detergent-OUT™ GBS10

Detergents are essential for protein solubility during protein extraction and sample preparation, especially

when working with hydrophobic proteins. The presence of high concentrations of detergents in protein

samples can impair ELISA, IEF, protease digestion of proteins, and suppress peptide ionization when

analyzed by mass spectrometry.

DetergentOUT™ GBS10 features a research grade detergent binding resin that has a high affinity for most

detergents and a low affinity for proteins and peptides. DetergentOUT™ GBS10 is ideal for detergent removal

prior to ELISA, IEF, protease digestion of proteins, and peptide ionization when analyzed by mass

spectrometry.

DetergentOUT™ GBS10 is available in four spin format column sizes or as resin alone.

The column specifications are:

Figure 1

Figure 2

Figure 3

Our DetergentOUT™ GBS10 resin removes free, unbound anionic, nonionic or zwitterionic detergents (e.g.

SDS, Triton? X-100 or CHAPS) from aqueous protein and peptide samples with minimal sample loss (Table

below) for downstream analysis by mass spectrometry (Figure 1 & 2) and other techniques.

The DetergentOUT™ GBS10 columns were shown in independent studies to be fully compatible with DI-QTOF

and LC-MS/MS (see references). The use of the DetergentOUT™ GBS10 columns significantly increased the

number of peptide spectra detected (Figure 1 & 2).

In addition, the DetergentOUT™ GBS10 columns have a high binding capacity for detergents, with 6 milligrams

SDS (Figure 3) and 14 milligrams Triton? X-100 for every milliliter of settled resin.

Detergent removal rates and percentage protein recovery with DetergentOUT™ GBS10

OrgoSol Detergent-OUT™

OrgoSol DetergentOUT™ is suitable for removal of detergents from protein solutions, including hydrophobic

protein solutions. OrgoSol DetergentOUT™ is compatible with all detergent types and its performance is not

dependent on the concentration of detergents in the solution. OrgoSol DetergentOUT™ is highly flexible and

can be used to process small and large sample volumes.

OrgoSol DetergentOUT™ first concentrates the protein solution through precipitation and then the detergent is

extracted and removed with the supplied OrgoSol™ buffer. The proprietary precipitation agent ensures >99%

protein recovery, however precipitation may result in some loss of a protein's biological activity.

Two sizes are offered for single or multiple experiments:

• Micro Kit for processing up to a total of 10ml protein solution

• Medi kit for processing up to a total of 30ml protein solution

REFERENCES

• Cortes, D.F. et al (2012) Electrophoresis. 33:3712

• Orr, S.J. et al (012) Molecular Sys Biol. DOI: 10.1038/msb.2012.5

• Troese, M.J. et al (2011) Infect Immunol. 79:4696

DetergentOUT™ Tween?

DetergentOUT™ Tween? is a spin column format detergent removal resin for polysorbate or Tween?

detergents or surfactants.

DetergentOUT™ Tween? removes polysorbate detergents without significant loss of proteins, dilution of the

protein solution, or change to the buffer composition of the protein solution.

For other detergents, we highly recommend our new DetergentOUT™ GBS10 columns and resin. The

DetergentOUT™ GBS10 shows greater efficiency of detergent removal and protein recovery for other

detergents, including SDS, CHAPS, Triton, Nonidet and Brij

Feature

• For the removal of Tween? detergents

REFERENCES

• Einsle, O. et al (2002) Acta Sryst. D58:341

• Fisher, J. and Margulies, S. (2002) Am J Physiol Lung Cell Mol Physiol 283:L737

• Baizman, E. et al (2000) Microbiol 146:3129

Instruction Sheet (PDF)

Catalog No.

Product Name

Size

786-156

DetergentOUT™ GB S10-3000

10 columns

786-159

DetergentOUT™ GB-S10, Resin

10ml resin

786-154

DetergentOUT™ GBS10-125

10 columns

786-157

DetergentOUT™ GBS-S10-5000

10 columns

786-155

DetergentOUT™ GB-S10-800

10 columns

Catalog No.

Product Name

Size

786-128

                        OrgoSol DetergentOUT™, Medi

30 ml

786-127

                        OrgoSol DetergentOUT™, Micro

10 ml

Catalog No.

Product Name

Size

786-215

                       DetergentOUT™ Tween?, Medi

10 columns

786-214

                       DetergentOUT™ Tween?, Micro

10 columns