|
Actin Binding Protein, Ezrin
Mediator of cell adhesion and migration.
 |
|
Ezrin is probably involved in connecting the cytoskeleton to the plasma membrane. In epithelial cells.
It is required for the formation of microvilli and membrane ruffles on the apical pole.
|
Actin Binding Protein
Description
|
Protein
|
|
Ezrin (Synonyms: villin-2)
|
|
Origin
|
|
human recombinant (untagged)
|
|
Molecular mass
|
|
70kDa
|
|
Protein description
|
|
Ezrin is probably participating in connecting the cytoskeleton to the plasma
membrane. Like many proteins. Ezrin is autoinhibited by a head-tail interaction.
|
|
Actin interaction
|
|
The C-ter binds to actin. PlP2 and membrane proteins, e.g. CD44.
|
Properties
|
Form
|
|
Lyophilized, ready-to-use
|
|
Quantity
|
|
50 µg
|
|
Buffer
|
|
150mM NaCl, 20mM Hepes pH 7.4, 1mM DTT and 5% sucrose, when reconstituted
with 50 µl ultrapure water to obtain a 1.0 mg/ml solution.
|
|
Purity & Activity
|
|
Purity:>96% by scanning densitometry from Coomassie G-250 stained SDS-Gels.
|
|
Purification notes
|
|
Ezrin was expressed as a GST-tag fusion protein in E. coli. After purification by
GST-Agarose and subsequent GST-tag cleavage. the pure protein was yielded by
gel filtration chromatography.
|
|
Protein conc.
|
|
Determined by OD280 (0.1%=0.963).
|
|
Storage instructions
|
|
Ezrin is stored at -70°C upon arrival will be stable in performance for at least
6 months from the date of purchase. The solubilized protein is kept on ice and
should be stored at -20°C. in glycerol or cryoprotect (Cat.#: 6011-01).
Avoid repeated freeze / thaw cycles.
|
|
Shipping conditions
|
|
At ambient temperature. Upon delivery store at -70°C.
|
|
Remarks
|
|
For Use in Research only. Not for Use in Human or Veterinary Diagnostical or
Therapeutical applications.
|
|