Products

UDP assay kit

The MicroMolar UDP Assay is for measurement of UDP concentrations in a sub-micromolar to low

micromolar range. Other nucleoside diphosphates (ADP, GDP and CDP) showed very little background.

Nucleoside triphosphates (ATP, UTP, GTP and CTP) and monophosphates (AMP, UMP, GMP and CMP) are

not detected.

The UDP assay is based on fluorescence measurement with emission at 535 nm and excitation at 485 nm.  It

is a high throughput assay using regular black 96-well or 384-well plates.  Micro-cuvettes may also be used

for detection of the fluorescence signals. The assay can be used to measure contamination of UDP in UTP

samples or monitor hydrolysis of UTP. It can also be used for measurement of enzyme activities that

generate UDP molecules.  The UDP assay is compatible with common reaction buffers with magnesium.  It

is not compatible with samples containing high concentrations of DNA or RNA.

MicroMolar UDP assay kit - 100 assays (Catalog number: MUD100K): The kit contains 10 x buffer, 100 x MUD

reagent 1, 100 x MUD reagent 2, 10 x fluorescence dye and 1 mM UDP control.  The assay reagents are

sufficient for measurement of 100 samples of UDP using a standard black 384-well plate.

MicroMolar UDP assay kit -500 assays (Catalog number: MUD500K): The kit contains 10 x buffer, 100 x MUD

reagent 1, 100 x MUD reagent 2, 10 x fluorescence dye and 1 mM UDP control.  The assay reagents are

sufficient for measurement of 500 samples of UDP using a standard black 384-well plate.