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Formalin-fixed and paraffin-embedded (FFPE) tissues are widely available and serve as a potential rich

source for biomedical research. However protein extraction from FFPE tissue is very challenging due to

formalin mediated molecular cross-linking. Traditional methods for protein extraction from FFPE tissues

involve repeated deparaffinization and rehydration using organic solvents followed by protein extraction at

high temperature with or without optional sonication step. These methods, thoughrelatively effective in some

cases, are tedious and time consuming (3-4 h). FE-025 provides a simple and rapid way to extract protein

from FFPE tissue without organic solvent deparaffinization. The whole procedure can be completed in less

than one hour with a protein yield of 2-3mg/ml.

Fgi. 1. Effect of Extraction time on protein yield and profile. Mouse kidney was fixed with formalin (one week)