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Epitope Tags, Anti-His-Tag Rabbit Monoclonal Antibody,
Clone RM146; 6x His-Tag or 10x His-Tag
RevMAb 사는 hybridoma를 사용하지 않고 monoclonal 항체 개발을 가능하게 하는 혁신적인 단클론 항체 제작
기술을 개발하여, 기존의 hybridoma 기술에서는 접근 할 수 없는 다양한 항원 특이적 B 세포에서 항체가
제작됩니다.
본 기술은 고품질의 rabbit monoclonal antibody와 goat monoclonal antibody 개발에 적용 할 수 있습니다.
세포융합법에 의한 hybridoma 제작 방법과는 달리, 항체는 ELISA와 westerblot 등 면역측정법에 의해 면역된
토끼나 염소의 B 세포에서 직접 screening됩니다. Target B 세포를 동정한 후 cDNA를 cloning 하고, 일과성
안정 포유 동물 세포 발현(transient and stable mammalian cell expression)을 이용하여 재조합 항체를
생산합니다.
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Fig. 1. Western blot of 293T cells:
(1) untransfected or
(2) transfected with His-Tag fusion protein X and
(3) E. coli lysate without His-Tag Protein Y
(4) E. coli lysate with His-Tag Protein Y
Using RevMAb Clone RM146 at 0.2 ug/mL.
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Product Details
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Product Name
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Anti-His-Tag (6x His-Tag or 10x His-Tag) Rabbit Monoclonal Antibody [RM146].
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Product Description
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Rabbit monoclonal to His-Tag. (6x His-Tag or 10x His-Tag) at either the
N-Terminus (amino) or C-Terminus (carboxy)
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Clone Name
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RM146
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Produt Data Sheet
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Anti-His Tag Rabbit Monoclonal Antibody Data Sheet RM146 (PDF)
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Specificity
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RM146 reacts to recombinant proteins containing the 6xHis-Tag or 10xHis-Tag
fused to either the amino or carboxy terminus. No cross reactivity with other
endogenous protein in mammalian or bacteria cells.
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Immunogen
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Mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with
6x His-Tag at the C-terminus.
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Application
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Immunohistochemistry
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Immunocytochemistry
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Western Blot
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Species Reactivity
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All
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Conjugate
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None
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Intended Use
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Research use only
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Properties
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Form
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Liquid
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Storage Instructions
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Store at 2-8°C
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Stability
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Two Years
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Storage Buffer
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PBS with 1% BSA and 0.09% sodium azide
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Volume/Item Size
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100ug
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Concentration
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1.0 mg/mL
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Purity
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Protein A affinity purified from an animal origin–free culture supernatant
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Clone Type
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Monoclonal
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I sotype
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Rabbit IgG
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Usage
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Western Blot: 0.1 ug/mL - 1 ug/mL;
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Immunoprecipitaion : 0.5 ug/mL - 2 ug/mL;
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Immunocytochemistry : 0.5 ug/mL - 2 ug/mL
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Flow Cytometry : 0.5 ug/mL - 2 ug/mL
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Fig. 2. mmunoprecipitation of 293T cells expressing
a His-Tag fusion protein by RevMAb clone RM146,
was blotted with RM146.
(1) Whole lysate control
(2) IP by rabbit IgG control
(3) IP by RM146.
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