Cellagen Technology LLC's new products
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Cellagen Technology LLC's new products
Repro-ACTH2i (1000x) (K2284) / Repro-nSC (1000x) (K6720) / Repro-ThiPSC (K8447)
Product Name: Repro-ACTH2i (1000x) (K2284)
ES cell lines can be established nondestructively from single blastomeres, and still retain their pluripotency. The inhibition of endogenous differentiation and activation of proliferation in preimplantation embryos is helpful in establishing ESC lines.
Studies have demonstrated that media containing 2i (PD0325901 and CHIR99021) dramatically increases the efficiency of
establishing mESCs(Ref.1). ACTH 1-24, adrenocorticotropic hormone fragments 1-24 also improved the survival/
proliferation of mESCs, and enhanced the derivation of mESCs from single blastomeres(Ref.2). When used together,
ACTH2i synergistically enhanced the derivation of mESC lines from ICR and C57BL/6J 0.5-4.5 days postcoitum denuded
intact zygotes, embryos, or isolated blastomeres from 2- to 8-cell embryos when cultured in small volume droplets in KSR
ESC medium with feeders. This method is easy to operate and highly efficient(Ref.3).
Details
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Cocktail Formulation:
1000xDMSO stock
solution |
10 mM of ACTH 1-24 peptide
3 mM of CHIR99021 (GSK3ß inhibitor), and
0.5 mM of PD0325901 (MEK inhibitor). | |
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Purity: |
>98% (for each compound) | |
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Storage: |
Store DMSO solution at -20°C.. | |
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Usage: |
Add/Dilute Repro-ACTH2i (1000x) at 1:1000 into your ESC media to make 1x Repro-ACTH2i media. Make the media fresh before each use. |
References
1. Ying QL, et al. The ground state of embryonic stem cell self-renewal. Nature. 2008. 453(7194):519-23.
2. Wakayama S, et al. Efficient establishment of mouse embryonic stem cell lines from single blastomeres and polar
bodies. Stem Cells. 2007. 25(4):986-93.
3. Lee KH, et al. Simple and efficient derivation of mouse embryonic stem cell lines using differentiation inhibitors or
proliferation stimulators. Stem Cells Dev. 2012. 21(3):373-83.
Ordering information
1: K2284-250 (0.25 mL of 1000x)
Product Name: Repro-nSC (1000x) (K6720)
Repro-nSC is a combination of two small molecules: a GSK-3ß inhibitor CHIR99021, and a TGF-ß receptor inhibitor SB431542. A study has shown the efficient conversion of hESCs into homogenous primitive neuroepithelium within 7 days when under
synergistic inhibition of GSK-3ß and TGF-ß pathways by Repro-nSC alone or in combination with inhibition of the
Notch signaling pathway (e.g. by RO4929097). These primitive neural stem cells can stably self-renew in the presence of
LIF and 1x Repro-nSC, retaining high neurogenic potential and responsiveness to instructive neural patterning cues
towards midbrain and hindbrain neuronal subtypes, in addition to exhibiting in vivo integration.
Details
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Cocktail Formulation:
1000xDMSO stock
solution |
12 mM of SB431542 (TGF-ß1 inhibitor)
3 mM of CHIR99021 (GSK3ß inhibitor) | |
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Purity: |
>98% (for each compound) | |
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Storage: |
Store DMSO solution at -20°C.. | |
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Usage: |
Dilute Repro-nSC stock solution (1000x) at 1:1000 into your neural induction media to make 1xRepro-nSC media. |
Reference
1. Li W., et al. Rapid induction and long-term self-renewal of primitive neural precursors from human embryonic stem cells
by small molecule inhibitors Proc Natl Acad Sci 2011 108(20):8299-304.
Ordering information
1: K6720-500 (0.5 mL of 1000x)
Product Name: Repro-ThiPSC (K8447)
Repro-ThiPSC (i.e. SMC4 in reference 1) is a cocktail of four small molecules of specific signaling pathway inhibitors. It can be added directly in media to increase reprogramming efficiency of somatic cells to iPSCs, and to improve expansion and
maintenance of hiPSCs under both with feeder cells and feeder-free conditions. It can be used in combination with any
of the widely used reprogramming gene expression systems (e.g. retro/lenti-viral, episomal, mRNA, or proteins) to
enhance the reprogramming somatic cells to iPS cells. This process is robust, efficient and can be easily scaled-up(Ref.1-3).
Details
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Cocktail Formulation:
1000xDMSO stock
solution |
5 mM of Thiazovivin (ROCK inhibitor),
0.4 mM of PD325901 (MEK inhibitor),
2 mM of SB431542 (TGF-ß1 inhibitor), and
1 mM of CHIR99021 (GSK3ß inhibitor) | |
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Purity: |
>98% (for each compound) | |
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Storage: |
Store DMSO solution at -20°C. | |
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Usage: |
Dilute Repro-ThiPSC stock solution (1000x) at 1:1000 into basic iPSC induction media
to make 1xRepro-ThiPSC media. |
References
1. Valamehr B, et al. A novel platform to enable the high-throughput derivation and characterization of feeder-free
human iPSCs. Scientific Reports. 2012. 2: 213.
2. Li W, et al. Generation of human-induced pluripotent stem cells in the absence of exogenous Sox2. Stem Cells. 2009.
27(12):2992-3000.
3. Kawamata M, et al. Generation of genetically modified rats from embryonic stem cells. PNAS. 2010. 107, 14223-
14228.
Ordering information
1: K8447-500 (0.5 mL of 1000x)
- 이전글Epigenetics / Cellagen Technology LLC 13.01.22
- 다음글StemEZ Serum-Free Medium (K3980) 12.10.27
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