Topoisomerase ATPase Assays
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Topoisomerase ATPase Assays
Type II DNA topoisomerases including topoisomerase II and topoisomerase IV hydrolyze ATP as the source of molecular
energy to carry out DNA supercoiling and DNA decatenation reactions. Inhibition of the ATPase activity of topoisomerases by compounds such as novobiocin blocks their biological functions. Therefore, the topoisomerase ATPase assay can be used for high-throughput screen of topoisomerase inhibitors. The Topoisomerase ATPase Assay Kit is based on
detection of the phosphate produced by the ATP hydrolysis reaction in the presence of DNA. The assay is in a 384-well
plate format and the phosphate is detected using light absorbance at 650 nm.(for 1000 assays ; enzyme is not included.)
TA1000 Topoisomerase ATPase Assay Kit
T2A-100KE E. coli gyrase ATPase assay Kit Plus (enzyme included) includes the reaction buffer, DNA, E. coli gyrase, ATP and the phosphate detection reagent for 100 assays of DNA supercoiling reactions in a 384-well assay format.
T2A-100KS S. aureus gyrase ATPase assay Kit Plus (enzyme included) includes the reaction buffer, DNA, S. aureus gyrase, ATP and the phosphate detection reagent for 100 assays of DNA supercoiling reactions in a 384-well assay
format.
T4A-100KE E. coli Topo IV ATPase Assay Kit Plus (enzyme included) includes the reaction buffer, DNA, E. coli Topo IV, ATP and the phosphate detection reagent for 100 assays of DNA supercoiling reactions in a 384-well assay format.
T4A-100KS S. aureus Topoisomerase IV ATPase Assay Kit Plus (enzyme included) includes the reaction buffer,
DNA,S. aureus Topoisomerase IV, ATP and the phosphate detection reagent for 100 assays of DNA supercoiling
reactions in a 384-well assay format.
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- 다음글Human topoisomerase II-DNA decatenation assay kits 12.11.04
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