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Human Neuron Cells (HNCs)

HNCs (cAP-0036) are initiated by digestion of minced brain cortical tissue with collagenase. HNCs are

separated from the mixture of cell populations and offered at passage 3 in a frozen vial. HNCs Growth

Medium (contains 10% serum and growth supplements, cAP-34) is recommended for cell culture and these

cells have a minimum average population doubling capacity > 8 when cultured following the detailed protocoldescribed below).

Characterization of the cells

The cells stain positively for a number of neuronal markers:

Neurofilament protein Positive

Neuron specific enolase (NSE) Positive

The cells are negative for

Glial fibrillary acidic protein (GFAP) Negative

Myelin basis protein (MBP) Negative

HNCs are negative for HIV-1, HBV, HCV, and mycoplasma