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Rat Endothelial Cells
Rat Aortic Endothelial cells
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rAECs (cAP-r0001) are isolated from aorta of adult SD rats. The cells are shipped in proliferating culture with
>90 confluence (the cells are provided @ passage 3). Endo- Growth Medium (cAP-02) is recommended for
the expansion of rAECs and these cells can be propagated to sixth passage and beyond without losing their
morphologic and phenotypic characteristics when cultured following the detailed protocol described below).
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Rat Brain Microvascular Endothelial Cells
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rBMVECs (cAP-r0002) are isolated from brain tissues of adult SD rats. The cells are shipped in proliferating
culture with >90 confluence (the cells are provided @ passage 3). Endo-Growth Medium (cAP-02) is
recommended for the expansion of rBMVECs and these cells can be propagated to sixth passage and
beyond without losing their morphologic and phenotypic characteristics when cultured following the detailed
protocol described below).
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Rat Cardiac Microvascular Endothelial Cells
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rCarMVECs (cAP-r0003) are isolated from heart tissues of adult SD rats. The cells are shipped in proliferating
culture with >90 confluence (the cells are provided @ passage 3). Endo-Growth Medium (cAP-02) is
recommended for the expansion of rCarMVECs and these cells can be propagated to sixth passage and
beyond without losing their morphologic and phenotypic characteristics when cultured following the detailed
protocol described below).
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Rat Pulmonary Microvascular Endothelial Cells
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RPulMVECs (cAP-r0004) are isolated from lung tissues of adult SD rats. The cells are shipped in proliferating
culture with >90 confluence (the cells are provided @ passage 3) or in a frozen vial. Rat Endothelial Growth
Medium (REGM, cAP-02r) is recommended for the expansion of RAECs and these cells can be propagated
to sixth passage and beyond without losing their morphologic and phenotypic characteristics when cultured
following the detailed protocol described below).
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Characterization of the cells
PECAM1: >95% positive by immunofluorescence
VE-Cadherin: >95% positive by immunofluorescence
negative for mycoplasma
RPulMVECs are tested negative for mycoplasma
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