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Living / Dead Viability_Cytotoxicity kit for mammalian cells
The Living/Dead Viability/Cytotoxicity Kit for Animal Cells provides a two-color fluorescence staining on both
live and dead cells using two probes that measure two recognized parameters of cell viability — intracellular
esterase activity and plasma membrane integrity. The kit is suitable for use with fluorescence microscopes,
fluorescence multiwell plate scanners and flow cytometers and other fluorescence detection systems. The
assay principles are general and applicable to most eukaryoticcell types, including adherent cells and
certain tissues, but not to bacteria or yeast. It is generally faster, less expensive, safer and a more sensitive
indicator of cytotoxic events than alternative methods. Live cells are distinguished by the presence of
ubiquitous intracellular esterase activity, determined by the enzymatic conversion of the virtually
nonfluorescent cell-permeant calcein AM to the intensely fluorescent calcein. The polyanionic dye calcein is
well retained within live cells, producing anintense uniform green fluorescence in live cells (Ex/Em ~495
nm/~520 nm). Propidium iodide (PI) enters cells with damaged membranes and undergoes a 30-fold
enhancement of fluorescence upon binding to nucleic acids, thereby producing a bright red fluorescence in
dead cells (Ex/Em ~528 nm/~617 nm). PI is excluded by the intact plasma membrane of live cells.
Applications;
Fluorescence Microscopy, Flow Cytometry
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