Products

Human DNA polymerase assay kits

Human DNA polymerase alpha assay kit

Human DNA Polymerase Alpha (Pol α) is responsible for the initiation of DNA replication at origins of

replication The Pol α enzyme consists of four subunits: the catalytic subunit POLA1, the regulatory subunit

POLA2, and the small and the large primase subunits PRIM1 and PRIM2.  The human DNA polymerase alpha

assay is based on measurement of the DNA molecules synthesized by the DNA polymerase.  The assay is

performed in a 384-well or 96-well plate format. The assay can be used for detection of DNA polymerase

alpha activity and high throughput screen of human DNA polymerase alpha inhibitors.  

The Human DNA Polymerase Alpha Assay Kit (Catalog No.  HDPA100K) includes all the assay kit

components except the enzyme for 100 assays in a 384-well plate assay format: 400 µl of 10 x Buffer, 33 µl of

100 x DNA template, 33 µl of 100 x dNTP  mix, 1550 µl of 2 x Dye and 1550 µl of 50 mM EDTA. The kit does

not include the enzyme.

The Human DNA Polymerase Alpha Assay Kit Plus (Catalog No.  HDPA100KE) includes all the assay kit

components for 100 assays in 384-well plate assay format: 400 µl of 10 x Buffer, 33 µl of 100 x DNA template,

33 µl of 100 x dNTP  mix, 33 µl of 100 x human DNA polymerase alpha,  1550 µl of 2 x Dye and 1550 µl of 50

mM EDTA.

Human DNA polymerase beta assay kit

DNA polymerase beta play key roles in the base excision repair (BER) process.  It fills the small gaps (1 – 6

bases) of one DNA stand using the complementary strand as a template.  Human DNA polymerase beta is

an anti-cancer target.  The human DNA polymerase beta assay is based on the principle that the repaired

DNA forms more stable DNA duplex than the un-repaired one.  The Assay Kit is to measure the formation of

the repaired DNA by its fluorescence signal in the presence of a fluorescence dye.

Each Human DNA Polymerase Beta Assay Kit (Catalog No: DPB100K)  includes 800 µl of 10 x Buffer BP, 55 µl

of 100x DNA template, 55 µl of 100 x dNTP mix, 22 ml of Reagent U and 420 µl of 10 x fluorescence dye for 100

assays of human DNA polymerase reactions in a 96-well plate format. The kit does not include human DNA

polymerase beta.

Each Human DNA Polymerase Beta assay Kit Plus (Catalog No: DPB100KE) includes 800 µl of 10 x Buffer

BP, 55 µl of 100x DNA template, 55 µl of 100 x dNTP mix, 22 ml of Reagent U, 52 µl of 100 x human DNA

polymerase beta and 420 µl of 10 x fluorescence dye for 100 assays of human DNA polymerase reactions in

a 96-well plate format.

Human DNA Polymerase Gamma assay kit

Human DNA polymerase gamma is the only DNA polymerase in human mitochondria and is responsible for

the DNA replication, recombination and repairing in human mitochondria. It plays critical roles in

mitochondrial diseases and aging. Some antiviral nucleoside analogs were reported to inhibit DNA

polymerase gamma after intracellular phosphorylation and cause severe chronic toxicity.  The human DNA

polymerase gamma assay is based on measurement of the DNA molecules synthesized by the DNA

polymerase.  The assay is performed in a 384-well or 96-well plate format. The assay can be used for

detection of DNA polymerase gamma activity and high throughput screen of human DNA polymerase gamma

inhibitors.

The Human DNA Polymerase Gamma Assay Kit (Catalog No.  DPG100K) includes all the assay kit

components except the enzyme for 100 assays in a 384-well plate assay format: 400 µl of 10 x Buffer, 33 µl of

100 x DNA template, 33 µl of 100 x dNTP  mix, 1550 µl of 2 x Dye, 1550 µl of 50 mM EDTA.

The Human DNA Polymerase Gamma Assay Kit Plus (Catalog No.  DPG100KE) includes all the assay kit

components for 100 assays in 384-well plate assay format: 400 µl of 10 x Buffer, 33 µl of 100 x DNA template,

33 µl of 100 x dNTP  mix, 33 µl of 100 x human DNA polymerase gamma,  1550 µl of 2 x Dye, 1550 µl of 50

mM EDTA.