Human iPSc & iPSc derivatives I
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Human iPSc & iPSc derivatives I
Induced pluripotent stem (iPS) cell technology offers unprecedented opportunity to deliver any kind of human body cells. It gives real promise to significantly advance fields of disease research, drug screening and development, cell and gene therapy, toxicity testing as well regenerative medicine. Celther Polska offers numerous differentiated human cells derived from human iPS cells.
Celther iPS cells (CLTH / iPS cells)
Immunofluorescent studies indicate expression of transcription factors (Sox2, Oct4, Nanog) as well as surface proteins (Tra1-81 and Tra1-60) characteristic for iPS cells. Furthermore, Celther iPS cells retain the ability to differentiate to cells originating from all three germ layers. Induced pluripotent stem cells have been derived from human dermal fibroblast.
Celther iNS cells (CLTH / iNS cells) Immunofluorescence studies indicate expression of NSC markers: Sox2 and Nestin. Under appropriate conditions (culture in medium with EGF and bFGF) iNS cells remain in undifferentiated state, whereas withdrawal of growth factors causes spontaneous differentiation to neurons and glial.
Cardiomyocytes Celther Induced Cardiomyocytes (CLTH / Induced Cardiomyocytes) Induced Cardiomyocytes are human cardiomyocytes derived from iPS cells through Celther proprietary differentiation protocols. Immunofluorescence studies indicate expression of the alpha-myosin heavy chain (Myh6). Induced Cardiomyocytes culture consist of beating areas containing contracting cardiomyocytes.These human cardiac cells are specifically designed to aid drug discovery and improve the predictability of drug efficacy and toxicity screens, weeding out ineffective and potentially toxic compounds early in the pharmaceutical pipeline process before significant time and resources have been invested.
Celther Astrocytes (CLTH / Astrocytes) Celther Human Astrocytes are normal human cells derived from human induced neural stem cells. Astrocytes are glial cells found in the brain and spinal cord that play a critical role in maintenance, support, and repair of nervous tissue. They regulate the extracellular ionic and chemical environment, respond to CNS injury, and play a fundamental role in the pathogenesis of ischemic neuronal death. When used together with Celther Astrocyte Medium, Celther Human Astrocytes are highly mature and differentiated GFAP positive cells with the typical star-like morphology. Normal Human Astrocytes (NHA) can be used to study the function of the central nervous system and how neural cells interact.
Celther Neurons (CLTH / Neurons) Celther has developed Celther human induced neurons derived from iPS cells. This readily and stably available human neurons will have enormous contribution to understanding the mechanism of human nervous system as well as provide a unique in vitro system for preclinical drug discovery, neurotoxicity testing, predictive disease modeling, and basic cellular research . Celther neural cell products and services can be flexibly tailored for the customers' needs by genetic manipulation, making patient-derived neurons or any other collaborations. These cells quickly assume a typical neuronal morphology with branching neurites. Antibodies to MAP2 are markers on neuronal cells, their perikarya and neuronal dendrites. In contrast tau is found predominantly in neuronal axons. Celther Neurons are both MAP2 and TAU positive in immunocytochemical staining. In addition, Celther neurons display a stable adherent single-cell morphology and remain viable for an extended culture period (>=14 days), making them amenable to a variety of electrophysiology, neurotoxicity, and synaptic neurotransmission assays. Easy to implement and available in commercial quantities, these high quality, highly pure neurons are are cryopreserved at passage one. Once thawed, Celther neurons remain viable for extended culture periods, allowing for acute and chronic studies.
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