CasPASE Apoptosis Colorimetric Assay™
본문
|
CasPASE Apoptosis Colorimetric Assay™
The CasPASE™ Apoptosis Colorimetric Assay provides a simple and easy to follow method for assaying caspases 1-10, a key early indicator of apoptosis in mammalian cells. The assay is based on the detection of cleavage of a synthetic substrate, which is labeled with the chromophore ρ-nitroaniline (ρNA) at the C-terminal. When liberated from the peptide, ρNA produces an optical change that can be detected by reading the absorbance at 405nm. The reaction is selectively and irreversibly inhibited by the peptide Z-VAD-FMK. Comparison of the absorbance of an induced / apoptotic sample with an uninduced control allows one to determine the fold- increase in protease activity. The Caspase activity can also be quantitated by using a standard curve established with the free ρNA dye. CasPASE™ assays are available for caspase enzymes 1 through 10. Each CasPASE™ assay kit is supplied with necessary assay buffers, enzyme specific ρNA-substrate, free dye (ρNA), and the potent caspase inhibitor Z-VAD-FMK for establishing proper positive and negative controls and standards.
Fluorescent Caspase Assays are also available.
Features • Assays for caspase enzymes 1 through 10 are available • Colorimetric detection (405nm) • Conveniently adaptable for high-throughout 96-well format • All kits supplied with enzyme specific (ρNA) & inhibitor Z-VAD-FMK
Applications • Monitor apoptosis by measuring various caspase activities in cells and tissues • Use with purified enzyme preparations, cell extracts, and tissue lysates • Characterization of caspase enzymes
Instruction sheet (pdf) |
Ordering informations
|
Catalog No. |
Product Name |
Size |
|
786-858A |
CasPASE™ 1, 4, 5 Assay with Ac-WEHD-pNA Substrate |
50 assays |
|
786-858B |
CasPASE™ 1, 4, 5 Assay with Ac-WEHD-pNA Substrate |
100 assays |
|
786-859A |
CasPASE™ 2 Assay with Ac-VDVAD-pNA Substrate |
50 assays |
|
786-859B |
CasPASE™ 2 Assay with Ac-VDVAD-pNA Substrate |
100 assays |
|
786-860A |
CasPASE™ 3, 7, 10 Assay with Ac-DEVD-pNA Substrate |
50 assays |
|
786-860B |
CasPASE™ 3, 7, 10 Assay with Ac-DEVD-pNA Substrate |
100 assays |
|
786-861A |
CasPASE™ 6 Assay with Ac-VEID-pNA Substrate |
50 assays |
|
786-861B |
CasPASE™ 6 Assay with Ac-VEID-pNA Substrate |
100 assays |
|
786-862A |
CasPASE™ 8 Assay with Ac-IETD-pNA Substrate |
50 assays |
|
786-862B |
CasPASE™ 8 Assay with Ac-IETD-pNA Substrate |
100 assays |
|
786-863A |
CasPASE™ 9 Assay with Ac-LEHD-pNA Substrate |
50 assays |
|
786-863B |
CasPASE™ 9 Assay with Ac-LEHD-pNA Substrate |
100 assays |
▣ 관련 페이지 ; G-Biosciences
댓글목록
등록된 댓글이 없습니다.